| Catalog Number | 11308-1-AP |
| Synonyms | GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2 |
| Host | Rabbit |
| Reactivity | human, canine and More (5) |
| Reactivity | human, canine, pig, monkey, zebrafish, hamster, sheep |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human spleen tissue, MCF-7 cells, Calu-1 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:20000-1:100000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human spleen tissue, MCF-7 cells, Calu-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, canine |
| Cited Reactivity | human, pig, monkey, zebrafish, hamster, sheep |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species |
| Full Name | golgi autoantigen, golgin subfamily a, 2 |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC014188 |
| Gene Symbol | GM130 |
| Gene ID (NCBI) | 2801 |
| RRID | AB_2115327 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IF protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| IHC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| WB protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
| human | IF Adv Mater Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration Authors - Zhuo Li View Article |
| Tatyana (Verified Customer) (06-15-2025) | Incubation at RT for 1 h in 5% goat serum/PBST. Very strong specific signal of Golgi apparatus. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: SH-SY5Y cells/human GOLGA2/GM130 Antibody Immunofluorescence validation (1:1000 dilution) in SH-SY5Y cells/human (Cat no:11308-1-AP) |
| Boyan (Verified Customer) (04-02-2023) | very good for WB. Applications: Western Blot |
| Stephen (Verified Customer) (10-01-2019) | RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA Applications: Immunofluorescence, Primary Antibody Dilution: 1:300 Cell Tissue Type: RPE (human) |
| Citations | 237 |
| Dilutions | WB : 1:20000-1:100000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635) Protocols Product Specific Protocols FC protocol for GOLGA2/GM130 antibody 11308-1-AP Download protocol IF protocol for GOLGA2/GM130 antibody 11308-1-AP Download protocol IHC protocol for GOLGA2/GM130 antibody 11308-1-AP Download protocol WB protocol for GOLGA2/GM130 antibody 11308-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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