EDC3 Polyclonal antibody 16486-1-AP proteintech
$149.00
In stock
SKU
16486-1-AP
| Catalog Number | 16486-1-AP |
| Synonyms | YJDC, PP844, LSM16 homolog, LSM16, hYjeF_N2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, MCF-7 cells, HeLa cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human kidney tissue, human heart tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HEK-293T cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human heart tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9608 Product name: Recombinant human EDC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-508 aa of BC021271 Sequence: RHNSWSSSSRHPNQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVFEEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSKEFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTLLGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLPNFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFLRDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGEHAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA Predict reactive species |
| Full Name | enhancer of mRNA decapping 3 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 508 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC021271 |
| Gene Symbol | EDC3 |
| Gene ID (NCBI) | 80153 |
| RRID | AB_2095946 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96F86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for EDC3 antibody 16486-1-AP | Download protocol |
| IHC protocol for EDC3 antibody 16486-1-AP | Download protocol |
| IP protocol for EDC3 antibody 16486-1-AP | Download protocol |
| WB protocol for EDC3 antibody 16486-1-AP | Download protocol |
| human | bioRxiv DCPS modulates TDP-43 mediated neurodegeneration through P-body regulation Authors - Yingzhi Ye View Article |
| mouse | IF PLoS Genet Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association Authors - Lauren G Chukrallah View Article |
| rat | WB Cell Rep Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes. Authors - Eva Riechert View Article |
| Aditya (Verified Customer) (11-01-2024) | great with ecl Applications: Immunohistochemistry |
| Citations | 8 |
| Dilutions | WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition
Journal: Cancer Res | Authors: Liang Fang | Species: human | Application: IF - Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association
Journal: PLoS Genet | Authors: Lauren G Chukrallah | Species: mouse | Application: IF - Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.
Journal: Cell Rep | Authors: Eva Riechert | Species: rat | Application: WB - DDX6 modulates P-body and stress granule assembly, composition, and docking
Journal: J Cell Biol | Authors: Nina Ripin | Species: human | Application: IF,WB - Praja2 controls P-body assembly and translation in glioblastoma by non-proteolytic ubiquitylation of DDX6
Journal: EMBO Rep | Authors: Emanuela Senatore | Species: human | Application: WB - DCPS modulates TDP-43 mediated neurodegeneration through P-body regulation
Journal: bioRxiv | Authors: Yingzhi Ye | Species: human
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
