EIF2B4 Polyclonal antibody 11332-1-AP proteintech
$149.00
In stock
SKU
11332-1-AP
| Catalog Number | 11332-1-AP |
| Synonyms | Translation initiation factor eIF2B subunit delta, EIF2Bdelta, EIF2BD, eIF2B GDP-GTP exchange factor subunit delta, EIF2B |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, RIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | MCF-7 cells, K-562 cells, HeLa cells, HL-60 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1875 Product name: Recombinant human EIF2B4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC001870 Sequence: MAAVAVAVREDSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKGEQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGDVILVYGCSSL Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa |
| Calculated Molecular Weight | 523 aa, 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC001870 |
| Gene Symbol | EIF2B4 |
| Gene ID (NCBI) | 8890 |
| RRID | AB_2277640 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| IHC protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| WB protocol for EIF2B4 antibody 11332-1-AP | Download protocol |
| human | WB Elife eIF2B conformation and assembly state regulate the integrated stress response. Authors - Michael Schoof View Article |
| Citations | 12 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:20-1:200 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- The HRI branch of the integrated stress response selectively triggers mitophagy
Journal: Mol Cell | Authors: Yogaditya Chakrabarty KD Validated | Species: human | Application: WB - Sugar phosphate activation of the stress sensor eIF2B.
Journal: Nat Commun | Authors: Qi Hao | Species: human | Application: WB - Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.
Journal: Elife | Authors: Carmela Sidrauski | Species: human | Application: WB - The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.
Journal: Elife | Authors: Yao Liang Wong | Application: WB - Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.
Journal: ACS Cent Sci | Authors: Xiaoyu Zhang | Species: human | Application: WB - eIF2B conformation and assembly state regulate the integrated stress response.
Journal: Elife | Authors: Michael Schoof | Species: human | Application: WB - The ubiquitin ligase Triad1 influences myeloid leukemogenesis by regulating the integrated stress response.
Journal: J Biol Chem | Authors: Hao Wang | Species: human | Application: WB - Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.
Journal: Biochem J | Authors: Noel C Wortham | Species: human | Application: WB - Paradoxical Sensitivity to an Integrated Stress Response Blocking Mutation in Vanishing White Matter Cells.
Journal: PLoS One | Authors: Yusuke Sekine | Application: WB - Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.
Journal: Elife | Authors: Carmela Sidrauski | Species: human | Application: WB - eIF2B conformation and assembly state regulate the integrated stress response.
Journal: Elife | Authors: Michael Schoof | Species: human | Application: WB - The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.
Journal: Elife | Authors: Yao Liang Wong | Application: WB
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