EIF3I Recombinant monoclonal antibody 85603-2-RR proteintech
$149.00
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SKU
85603-2-RR
| Catalog Number | 85603-2-RR |
| Synonyms | eIF 3 beta, eIF3 beta, eIF3 p36, eIF-3-beta, EIF3S2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, Jurkat cells, HEK-293T cells, HepG2 cells, NIH/3T3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | HeLa cells, Jurkat cells, HEK-293T cells, HepG2 cells, NIH/3T3 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag1847 Product name: Recombinant human EIF3I protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC000413 Sequence: MKPILLQGHERSITQIKYNREGDLLFTVAKDPIVNVWYSVNGERLGTYMGHTGAVWCVDADWDTKHVLTGSADNSCRLWDCETGKQLALLKTNSAVRTCGFDFGGNIIMFSTDKQMGYQCFVSFFDLRDPSQIDNNEPYMKIPCNDSKITSAVWGPLGECIIAGHESGELNQYSAKSGEVLVNVKEHSRQINDIQLSRDMTMFVTASKDNTAKLFDSTTLEHQKTFRTERPVNSAALSPNYDHVVLGGGQEAMDVTTTSTRIGKFEARFFHLAFEEEFGRVKGHFGPINSVAFHPDGKSYSSGGEDGYVRIHYFDPQYFEFEFEA Predict reactive species |
| Full Name | eukaryotic translation initiation factor 3, subunit I |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC000413 |
| Gene Symbol | EIF3I |
| Gene ID (NCBI) | 8668 |
| RRID | AB_3744231 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13347 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for EIF3I antibody 85603-2-RR | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 243040B3 |
| Conjugation | Unconjugated |
Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex. Protocols Product Specific Protocols WB protocol for EIF3I antibody 85603-2-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- CAAP1 suppresses tumor growth and metastasis via interacting with EIF3I in high-grade serous ovarian carcinoma.
Journal: Am J Cancer Res | Authors: Chunyan Wang
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