Calpastatin Monoclonal antibody 67107-1-Ig proteintech
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67107-1-Ig
| Catalog Number | 67107-1-Ig |
| Synonyms | CAST, 1G12A2, Calpain inhibitor, Sperm BS-17 component |
| Host | Mouse |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, MCF-7 cells, THP-1 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, MCF-7 cells, THP-1 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28669 Product name: Recombinant human CAST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC013579 Sequence: MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKEEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK Predict reactive species |
| Full Name | calpastatin |
| Calculated Molecular Weight | 667 aa, 72 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC013579 |
| Gene Symbol | Calpastatin |
| Gene ID (NCBI) | 831 |
| RRID | AB_2882411 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P20810 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Calpastatin antibody 67107-1-Ig | Download protocol |
| IF protocol for Calpastatin antibody 67107-1-Ig | Download protocol |
| WB protocol for Calpastatin antibody 67107-1-Ig | Download protocol |
| mouse | WB Pharmacol Res Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD. Authors - Juan Wang View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:6000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1G12A2 |
| Conjugation | Unconjugated |
Calpastatin (CAST) is an endogenous protein that specifically inhibits the activity of calpains, a family of calcium-dependent proteases involved in numerous cellular processes. The CAST gene encodes calpastatin, which contains multiple calpain-inhibiting domains that bind to the active site of calpain, preventing it from performing its functions. Calpastatin is found in the cytosol and plays a key role in regulating intracellular proteolysis, with malfunctions in the calpain-calpastatin system linked to various diseases, including autoimmune disorders and neurodegenerative conditions Protocols Product Specific Protocols FC protocol for Calpastatin antibody 67107-1-Ig Download protocol IF protocol for Calpastatin antibody 67107-1-Ig Download protocol WB protocol for Calpastatin antibody 67107-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.
Journal: Oxid Med Cell Longev | Authors: Fengming Shen | Species: mouse | Application: WB - Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
Journal: Pharmacol Res | Authors: Juan Wang | Species: mouse | Application: WB
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