F4/80 Recombinant monoclonal antibody 84538-1-RR proteintech
$149.00
In stock
SKU
84538-1-RR
| Catalog Number | 84538-1-RR |
| Synonyms | Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1 |
| Host | Rabbit |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Positive IHC detected in | mouse spleen tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, mouse liver tissue |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Tested Reactivity | mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
| Calculated Molecular Weight | 102 kDa |
| GenBank Accession Number | NM_010130.4 |
| Gene Symbol | F4/80 |
| Gene ID (NCBI) | 13733 |
| RRID | AB_3743582 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q61549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for F4/80 antibody 84538-1-RR | Download protocol |
| IHC protocol for F4/80 antibody 84538-1-RR | Download protocol |
| Citations | - |
| Dilutions | IHC : 1:2000-1:8000 IF-P : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241959B6 |
| Conjugation | Unconjugated |
Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages. Protocols Product Specific Protocols IF protocol for F4/80 antibody 84538-1-RR Download protocol IHC protocol for F4/80 antibody 84538-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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