FUT9 Monoclonal antibody 60230-1-Ig proteintech
$149.00
In stock
SKU
60230-1-Ig
| Catalog Number | 60230-1-Ig |
| Synonyms | Alpha (1,3) fucosyltransferase, Fuc TIX, Fucosyltransferase 9, Fucosyltransferase IX, FucT IX, FUT9 |
| Host | Mouse |
| Reactivity | human, pig, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human breast cancer tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | pig brain tissue, rat brain tissue, mouse brain tissue, fetal human brain tissue |
| Positive IHC detected in | human cervical cancer tissue, human breast cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, pig, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8298 Product name: Recombinant human FUT9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 32-359 aa of BC036101 Sequence: PTNSWIFSPMESASSVLKMKNFFSTKTDYFNETTILVWVWPFGQTFDLTSCQAMFNIQGCHLTTDRSLYNKSHAVLIHHRDISWDLTNLPQQARPPFQKWIWMNLESPTHTPQKSGIEHLFNLTLTYRRDSDIQVPYGFLTVSTNPFVFEVPSKEKLVCWVVSNWNPEHARVKYYNELSKSIEIHTYGQAFGEYVNDKNLIPTISACKFYLSFENSIHKDYITEKLYNAFLAGSVPVVLGPSRENYENYIPADSFIHVEDYNSPSELAKYLKEVDKNNKLYLSYFNWRKDFTVNLPRFWESHACLACDHVKRHQEYKSVGNLEKWFWN Predict reactive species |
| Full Name | fucosyltransferase 9 (alpha (1,3) fucosyltransferase) |
| Calculated Molecular Weight | 359 aa, 42 kDa |
| Observed Molecular Weight | 44-46 kDa |
| GenBank Accession Number | BC036101 |
| Gene Symbol | FUT9 |
| Gene ID (NCBI) | 10690 |
| RRID | AB_11183046 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y231 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| IHC protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| WB protocol for FUT9 antibody 60230-1-Ig | Download protocol |
| mouse | WB Cancers (Basel) FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State. Authors - Athanasios Blanas KO Validated View Article |
| human | WB,IHC Sci Rep Integrative multi-omics analysis identified FUT9 and MS4A3 as novel immune-phenotype and prognosis biomarkers for colorectal cancer and analyze the role of FUT9 in oncoimmunology. Authors - Minjing Zhu KD Validated View Article |
| Citations | 3 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 5D4G6 |
| Conjugation | Unconjugated |
FUT9 is the main enzyme responsible for the synthesis of Lewis X (Lex) and catalyzes the last step in the biosynthesis of Lewis X antigen by addition of a fucose residue to precursor glycan structures. FUT9 is expressed in stomach, kidney, brain, and in leukocytes. Protocols Product Specific Protocols IF protocol for FUT9 antibody 60230-1-Ig Download protocol IHC protocol for FUT9 antibody 60230-1-Ig Download protocol WB protocol for FUT9 antibody 60230-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- FUT9-Driven Programming of Colon Cancer Cells towards a Stem Cell-Like State.
Journal: Cancers (Basel) | Authors: Athanasios Blanas KO Validated | Species: mouse | Application: WB - ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9
Journal: Acta Biochim Biophys Sin (Shanghai) | Authors: Aiping Xu KD Validated | Species: human | Application: WB,IHC - Integrative multi-omics analysis identified FUT9 and MS4A3 as novel immune-phenotype and prognosis biomarkers for colorectal cancer and analyze the role of FUT9 in oncoimmunology.
Journal: Sci Rep | Authors: Minjing Zhu KD Validated | Species: human | Application: WB,IHC
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