FAM162A Polyclonal antibody 32034-1-AP proteintech
$149.00
In stock
SKU
32034-1-AP
| Catalog Number | 32034-1-AP |
| Synonyms | C3orf28, DC16, E2IG5, E2-induced gene 5 protein, FWP001 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Caki-2 cells, HepG2 cells, RT-4 cells |
| Tested Applications — Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | Caki-2 cells, HepG2 cells, RT-4 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag36569 Product name: Recombinant human FAM162A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC015060 Sequence: MGSLSGLRLAAGSCFRLCERDVSSSLRLTRSSDLKRINGFCTKPQESPGVPSRTYNRVPLHKPTDWQKKILIWSGRFKKEDEIPETVSLEMLDAAKNKMRVKISYLMIALTVVGCIFMVIEGKKAAQRHETLTSLNLEKKARLKEEAAMKAKTE Predict reactive species |
| Full Name | family with sequence similarity 162, member A |
| Calculated Molecular Weight | 154 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC015060 |
| Gene Symbol | FAM162A |
| Gene ID (NCBI) | 26355 |
| RRID | AB_3670175 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96A26 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FAM162A antibody 32034-1-AP | Download protocol |
| IHC protocol for FAM162A antibody 32034-1-AP | Download protocol |
| WB protocol for FAM162A antibody 32034-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:200-1:800 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020). Protocols Product Specific Protocols IF protocol for FAM162A antibody 32034-1-AP Download protocol IHC protocol for FAM162A antibody 32034-1-AP Download protocol WB protocol for FAM162A antibody 32034-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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