| Catalog Number | 33047-1-AP |
| Synonyms | KIAA0592, Vaccinia virus penetration factor, VPEF, WASH complex subunit 2C, WASHC2C |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, HuH-7 cells, L02 cells |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HepG2 cells, HuH-7 cells, L02 cells |
| Positive IF/ICC detected in | A431 cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15066 Product name: Recombinant human FAM21C protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1036-1318 aa of BC006456 Sequence: MSVPRGPIAQWADGAISPNGHRPQLRAASGEDSTEEALAAAAAPWEGGPVPGVDTSPFAKSLGHSRGEADLFDSGDIFSTGTGSQSVERTKPKAKIAENPANPPVGGKAKSPMFPALGEASSDDDLFQSAKPKPAKKTNPFPLLEDEDDLFTDQKVKKNETKSSSQQDVILTTQDIFEDDIFATEAIKPSQKTREKEKTLESNLFDDNIDIFADLTVKPKEKSKKKVEAKSIFDDDMDDIFSTGIQAKTTKPKSRSAQAAPEPRFEHKVSNIFDDPLNAFGGQ Predict reactive species |
| Full Name | family with sequence similarity 21, member C |
| Calculated Molecular Weight | 1320 aa, 145 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC006456 |
| Gene Symbol | FAM21C |
| Gene ID (NCBI) | 253725 |
| RRID | AB_3742836 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y4E1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FAM21C antibody 33047-1-AP | Download protocol |
| WB protocol for FAM21C antibody 33047-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:6000 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
WASHC2C encodes WASH complex subunit 2C, a core subunit of the WASH complex that organizes endosomal membrane remodeling and cargo recycling. It binds phospholipids such as PtdIns-(4)P and the retromer component VPS35, linking endosomal localization to actin-based membrane dynamics. The protein is found on the early endosome membrane, in the early endosome, endosome, and cytosol, and is expressed at high levels in the male reproductive system with detectable expression in brain, endocrine system, and hematopoietic system. Protocols Product Specific Protocols IF protocol for FAM21C antibody 33047-1-AP Download protocol WB protocol for FAM21C antibody 33047-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols