FARS2 Polyclonal antibody 16436-1-AP proteintech
$149.00
In stock
SKU
16436-1-AP
| Catalog Number | 16436-1-AP |
| Synonyms | EC:6.1.1.20, FARS1, HSPC320, Phenylalanine tRNA ligase, Phenylalanine--tRNA ligase, mitochondrial |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells |
| Tested Applications — Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HeLa cells, Jurkat cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9750 Product name: Recombinant human FARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 109-451 aa of BC021112 Sequence: LFSVYDNLSPVVTTWQNFDSLLIPADHPSRKKGDNYYLNRTHMLRAHTSAHQWDLLHAGLDAFLVVGDVYRRDQIDSQHYPIFHQLEAVRLFSKHELFAGIKDGESLQLFEQSSRSAHKQETHTMEAVKLVEFDLKQTLTRLMAHLFGDELEIRWVDCYFPFTHPSFEMEINFHGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMILYDIPDIRLFWCEDERFLKQFCVSNINQKVKFQPLSKYPAVINDISFWLPSENYAENDFYDLVRTIGGDLVEKVDLIDKFVHPKTHKTSHCYRITYRHMERTLSQREVRHIHQALQEAAVQLLGVEGRF Predict reactive species |
| Full Name | phenylalanyl-tRNA synthetase 2, mitochondrial |
| Calculated Molecular Weight | 451 aa, 52 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC021112 |
| Gene Symbol | FARS2 |
| Gene ID (NCBI) | 10667 |
| RRID | AB_2102499 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FARS2 antibody 16436-1-AP | Download protocol |
| IHC protocol for FARS2 antibody 16436-1-AP | Download protocol |
| WB protocol for FARS2 antibody 16436-1-AP | Download protocol |
| human | WB Eur J Hum Genet Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival. Authors - Giulia Barcia View Article |
| rat,mouse,human | WB Nat Commun Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. Authors - Qian Zhou View Article |
| Citations | 5 |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
FARS2, a nuclear gene located on chromosome 6 (6p25.1), encodes the mitochondrial phenylalanyl-tRNA synthetase (mtPheRS), which transfers phenylalanine (Phe) to its cognate tRNA in mitochondria (PMID: 32774346). Protocols Product Specific Protocols IF protocol for FARS2 antibody 16436-1-AP Download protocol IHC protocol for FARS2 antibody 16436-1-AP Download protocol WB protocol for FARS2 antibody 16436-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Clinical and molecular characterization of novel FARS2 variants causing neonatal mitochondrial disease
Journal: Mol Genet Metab | Authors: Wenqian Chen | Species: human | Application: WB - New insights into the phenotype of FARS2 deficiency.
Journal: Mol Genet Metab | Authors: Elise Vantroys | Species: human | Application: WB - Mutation of the human mitochondrial phenylalanine-tRNA synthetase causes infantile-onset epilepsy and cytochrome c oxidase deficiency.
Journal: Biochim Biophys Acta | Authors: Abdulraheem Almalki | Species: human | Application: WB - Novel FARS2 variants in patients with early onset encephalopathy with or without epilepsy associated with long survival.
Journal: Eur J Hum Genet | Authors: Giulia Barcia | Species: human | Application: WB - Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ.
Journal: Nat Commun | Authors: Qian Zhou | Species: rat,mouse,human | Application: WB
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