FAT1 Recombinant monoclonal antibody 84279-3-RR proteintech
$149.00
In stock
SKU
84279-3-RR
| Catalog Number | 84279-3-RR |
| Synonyms | 241602F9, CDHF7, FAT, hFat1, ME5 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag33957 Product name: Recombinant human FAT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 4390-4550 aa of Sequence: PSVPLPDIQEFPNYEVIDEQTPLYSADPNAIDTDYYPGGYDIESDFPPPPEDFPAADELPPLPPEFSNQFESIHPPRDMPAAGSLGSSSRNRQRFNLNQYLPNFYPLDMSEPQTKGTGENSTCREPHAPYPPGYQRHFEAPAVESMPMSVYASTASCSDVS Predict reactive species |
| Full Name | FAT tumor suppressor homolog 1 (Drosophila) |
| Observed Molecular Weight | 506-600 kDa, 85 kDa |
| Gene Symbol | FAT1 |
| Gene ID (NCBI) | 2195 |
| RRID | AB_3671825 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14517 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for FAT1 antibody 84279-3-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241602F9 |
| Conjugation | Unconjugated |
FAT1, also known as hFat1, belongs to a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. It is expected to be located in cell membrane and nucleus, which is expressed in many epithelial and some endothelial and smooth muscle cells. The calculated molecular weight of FAT1 is 506 kDa and there is glycosylation modification of the protein. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK-293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction (PMID: 15922730, 26373379). The high molecular mass band observed at the expected 500-kDa size of FAT1 is indicated by an arrow together with a prominent band at 85 kDa (PMID: 21680732). Protocols Product Specific Protocols WB protocol for FAT1 antibody 84279-3-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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