Fibulin 5 Polyclonal antibody 12188-1-AP proteintech
$149.00
In stock
SKU
12188-1-AP
| Catalog Number | 12188-1-AP |
| Synonyms | ARMD3, DANCE, EVEC, FBLN5, FIBL 5, fibulin 5, UP50, Urine p50 protein |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue |
| Tested Applications — Positive IP detected in | mouse heart tissue |
| Tested Applications — Positive IHC detected in | mouse lung tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse heart tissue, rat brain tissue, mouse placenta tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse lung tissue, human heart tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2859 Product name: Recombinant human FBLN5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 186-448 aa of BC022280 Sequence: GSYSCTCNPGFTLNEDGRSCQDVNECATENPCVQTCVNTYGSFICRCDPGYELEEDGVHCSDMDECSFSEFLCQHECVNQPGTYFCSCPPGYILLDDNRSCQDINECEHRNHTCNLQQTCYNLQGGFKCIDPIRCEEPYLRISDNRCMCPAENPGCRDQPFTILYRDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPREIQLDLEMITVNTVINFRGSSVIRLRIYVSQYPF Predict reactive species |
| Full Name | fibulin 5 |
| Calculated Molecular Weight | 448 aa, 50 kDa |
| Observed Molecular Weight | 55 kDa, 65 kDa |
| GenBank Accession Number | BC022280 |
| Gene Symbol | Fibulin 5 |
| Gene ID (NCBI) | 10516 |
| RRID | AB_2105939 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| IP protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| WB protocol for Fibulin 5 antibody 12188-1-AP | Download protocol |
| mouse | WB Am J Physiol Cell Physiol Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function. Authors - Varadarajan Sudhahar View Article |
| human | WB Clin Exp Pharmacol Physiol Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway. Authors - Wei Zhang KD Validated View Article |
| Citations | 13 |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50 kDa or 66 kDa band in western blotting, the 66 kDa corresponding to fully glycosylated protein. Protocols Product Specific Protocols IHC protocol for Fibulin 5 antibody 12188-1-AP Download protocol IP protocol for Fibulin 5 antibody 12188-1-AP Download protocol WB protocol for Fibulin 5 antibody 12188-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Increased aortic stiffness and attenuated lysyl oxidase activity in obesity.
Journal: Arterioscler Thromb Vasc Biol | Authors: Chen Ju-Yi JY | Species: mouse | Application: WB - PPARγ activation improves the microenvironment of perivascular adipose tissue and attenuates aortic stiffening in obesity.
Journal: J Biomed Sci | Authors: Ju-Yi Chen | Application: WB - Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.
Journal: Biochem J | Authors: Kapustin Alexander A | Species: human | Application: WB - Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.
Journal: Cell Tissue Res | Authors: Wenli Han | Species: mouse | Application: WB, IHC - Caveolin-1 stabilizes ATP7A, Cu transporter for Extracellular SOD, in Vascular Tissue to Maintain Endothelial Function.
Journal: Am J Physiol Cell Physiol | Authors: Varadarajan Sudhahar | Species: mouse | Application: WB - Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.
Journal: Clin Exp Pharmacol Physiol | Authors: Wei Zhang KD Validated | Species: human | Application: WB - The matricellular protein Fibulin-5 regulates β-cell proliferation in an autocrine/paracrine manner
Journal: iScience | Authors: Tomoko Okuyama | Species: mouse | Application: WB - Fibulin-5 binds urokinase-type plasminogen activator and mediates urokinase-stimulated β1-integrin-dependent cell migration.
Journal: Biochem J | Authors: Kapustin Alexander A | Species: human | Application: WB - A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer
Journal: Mol Carcinog | Authors: Feiyu Qin | Species: human | Application: WB,IHC - Aberrant elastin remodeling in the lungs of O2-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin.
Journal: Cell Tissue Res | Authors: Wenli Han | Species: mouse | Application: WB, IHC - Fibulin-4 Accelerates Amyloid Formation by Binding with a Keratin 5 Peptide Fragment.
Journal: JID Innov | Authors: Fumihiko Katagiri | Species: human | Application: IF - Limb remote ischemic postconditioning-induced elevation of fibulin-5 confers neuroprotection to rats with cerebral ischemia/reperfusion injury: activation of the AKT pathway.
Journal: Clin Exp Pharmacol Physiol | Authors: Wei Zhang | Species: human | Application: WB - Transforming Growth Factor-β-Neutralizing Antibodies Improve Alveolarization in the Oxygen-Exposed Newborn Mouse Lung.
Journal: J Interferon Cytokine Res | Authors: Sijun Deng | Species: mouse | Application: WB
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