FBXL14 Polyclonal antibody 13934-1-AP proteintech
$149.00
In stock
SKU
13934-1-AP
| Catalog Number | 13934-1-AP |
| Synonyms | FBL14, F-box and leucine-rich repeat protein 14, F-box/LRR-repeat protein 14 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4975 Product name: Recombinant human FBXL14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-418 aa of BC028132 Sequence: METHISCLFPELLAMIFGYLDVRDKGRAAQVCTAWRDAAYHKSVWRGVEAKLHLRRANPSLFPSLQARGIRRVQILSLRRSLSYVIQGMANIESLNLSGCYNLTDNGLGHAFVQEIGSLRALNLSLCKQITDSSLGRIAQYLKGLEVLELGGCSNITNTGLLLIAWGLQRLKSLNLRSCRHLSDVGIGHLAGMTRSAAEGCLGLEQLTLQDCQKLTDLSLKHISRGLTGLRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFCDKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLELIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMTDSEKEARGDFSPLFTVRTRGSSRR Predict reactive species |
| Full Name | F-box and leucine-rich repeat protein 14 |
| Calculated Molecular Weight | 418 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC028132 |
| Gene Symbol | FBXL14 |
| Gene ID (NCBI) | 144699 |
| RRID | AB_10547838 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N1E6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FBXL14 antibody 13934-1-AP | Download protocol |
| IHC protocol for FBXL14 antibody 13934-1-AP | Download protocol |
| mouse,human | Cell Death Dis F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7. Authors - Y Liu View Article |
| human | Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells Authors - Christopher Noyes View Article |
| Citations | 3 |
| Dilutions | IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
FBXL14(F-box/LRR-repeat protein 14) is also named as FBL14 and belongs to the the F-box protein family,which are characterized by an approximately 40-amino acid F-box motif. It is part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. SNAIL1 is an unstable transcription factor that is phosphorylated by GSK3-beta in the nucleus, which triggers its nuclear export and subsequent ubiquitination and proteasomal degradation. FBXL14 is directed ubiquitination and degradation of SNAIL1 in human and mouse cell lines(PMID:19955572). Protocols Product Specific Protocols IF protocol for FBXL14 antibody 13934-1-AP Download protocol IHC protocol for FBXL14 antibody 13934-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- F-box protein Fbxl18 mediates polyubiquitylation and proteasomal degradation of the pro-apoptotic SCF subunit Fbxl7.
Journal: Cell Death Dis | Authors: Y Liu | Species: mouse,human - The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
Journal: Commun Biol | Authors: Christopher Noyes | Species: human - The deubiquitinase USP4 stabilizes the SCF(FBXO24) ubiquitin E3 ligase to regulate immunity in experimental pneumonia.
Journal: Nat Commun | Authors: Ilknur Yurtsever
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