FBXO27 Polyclonal antibody 14570-1-AP proteintech
$149.00
In stock
SKU
14570-1-AP
| Catalog Number | 14570-1-AP |
| Synonyms | FBG5, F-box only protein 27, F-box/G-domain protein 5, FBX27 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PANC-1 cells |
| Tested Applications — Positive IP detected in | PANC-1 cells |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | PANC-1 cells |
| Positive IP detected in | PANC-1 cells |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6157 Product name: Recombinant human FBXO27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC030060 Sequence: MGASVSRGRAARVPAPEPEPEEALDLSQLPPELLLVVLSHVPPRTLLGRCRQVCRGWRALVDGQALWLLILARDHGATGRALLHLARSCQSPARNARPCPLGRFCARRPIGRNLIRNPCGQEGLRKWMVQHGGDGWVVEENRTTVPGAPSQTCFVTSFSWCCKKQVLDLEEEGLWPELLDSGRIEICVSDWWGARHDSGCMYRLLVQLLDANQTVLDKFSAVPDPIPQWNNNACLHVTHVFSNIKMGVRFVSFEHRGQDTQFWAGHYGARVTNSSVIVRVRLS Predict reactive species |
| Full Name | F-box protein 27 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC030060 |
| Gene Symbol | FBXO27 |
| Gene ID (NCBI) | 126433 |
| RRID | AB_2104422 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NI29 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for FBXO27 antibody 14570-1-AP | Download protocol |
| IP protocol for FBXO27 antibody 14570-1-AP | Download protocol |
| human | WB,IF Proc Natl Acad Sci U S A Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy. Authors - Yukiko Yoshida KO Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
FBXO27 is a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and SCFFBXO27 ubiquitinates glycoproteins in damaged lysosomes will regulate autophagic machinery recruitment (PMID: 28743755).Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes. Protocols Product Specific Protocols IHC protocol for FBXO27 antibody 14570-1-AP Download protocol IP protocol for FBXO27 antibody 14570-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- TRIM16-mediated lysophagy suppresses high-glucose-accumulated neuronal Aβ
Journal: Autophagy | Authors: Chang Woo Chae | Species: human | Application: WB,IF - Ubiquitination of exposed glycoproteins by SCF(FBXO27) directs damaged lysosomes for autophagy.
Journal: Proc Natl Acad Sci U S A | Authors: Yukiko Yoshida KO Validated | Species: human | Application: WB,IF
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