FBXW2 Polyclonal antibody 11499-1-AP proteintech
$149.00
In stock
SKU
11499-1-AP
| Catalog Number | 11499-1-AP |
| Synonyms | F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, FBW2, FBXW 2, FWD2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2045 Product name: Recombinant human FBXW2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC018738 Sequence: MERKDFETWLDNISVTFLSLTDLQKNETLDHLISLSGAVQLRHLSNNLETLLKRDFLKLLPLELSFYLLKWLDPQTLLTCCLVSKQWNKVISACTEVWQTACKNLGWQIDDSVQDALHWKKVYLKAILRMKQLEDHEAFETSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCVYGIQTHTCAAVKFDEQKLVTGSFDNTVACWEWSSGARTQHFRGHTGAVFSVDYNDELDILVSGSADFTVKVWALSAGTCLNTLTGHTEWVTKVVLQKCKVKSLLHSPGDYILLSADKYEIKIWPIGREINCKC Predict reactive species |
| Full Name | F-box and WD repeat domain containing 2 |
| Calculated Molecular Weight | 44 kDa, 52 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC018738 |
| Gene Symbol | FBXW2 |
| Gene ID (NCBI) | 26190 |
| RRID | AB_2102740 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| WB protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| human | IP J Biol Chem USP2 is an SKP2 deubiquitylase that stabilizes both SKP2 and its substrates. Authors - Fengwu Zhang View Article |
| mouse,human | WB,IHC Cell Death Dis FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis Authors - Ganesh Kumar Barik KD Validated View Article |
| Citations | 8 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both. Protocols Product Specific Protocols IHC protocol for FBXW2 antibody 11499-1-AP Download protocol WB protocol for FBXW2 antibody 11499-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
Journal: Nat Commun | Authors: Jie Xu KD Validated | Species: human | Application: WB - FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2
Journal: Cancer Lett | Authors: Liyun Zheng | Species: human | Application: WB - TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.
Journal: Cell Mol Gastroenterol Hepatol | Authors: Shunjie Xia KD Validated | Species: human | Application: WB - FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradation.
Journal: Cell Mol Life Sci | Authors: Tao Zhou KD Validated | Species: human | Application: WB - USP2 is an SKP2 deubiquitylase that stabilizes both SKP2 and its substrates.
Journal: J Biol Chem | Authors: Fengwu Zhang | Species: human | Application: IP - FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis
Journal: Cell Death Dis | Authors: Ganesh Kumar Barik KD Validated | Species: mouse,human | Application: WB,IHC
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