FCGR3B/CD16b Polyclonal antibody 30201-1-AP proteintech
$149.00
In stock
SKU
30201-1-AP
| Catalog Number | 30201-1-AP |
| Synonyms | CD16, CD16b, FCGR3B, Fc gamma RIII, Fc gamma RIII beta |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Ramos cells, U-937 cells |
| Tested Applications — Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | Ramos cells, U-937 cells |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | Fc fragment of IgG, low affinity IIIb, receptor (CD16b) |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | NM_000570 |
| Gene Symbol | FCGR3B |
| Gene ID (NCBI) | 2215 |
| RRID | AB_3086262 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75015 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FCGR3B/CD16b antibody 30201-1-AP | Download protocol |
| IHC protocol for FCGR3B/CD16b antibody 30201-1-AP | Download protocol |
| WB protocol for FCGR3B/CD16b antibody 30201-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:200-1:800 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b. Protocols Product Specific Protocols IF protocol for FCGR3B/CD16b antibody 30201-1-AP Download protocol IHC protocol for FCGR3B/CD16b antibody 30201-1-AP Download protocol WB protocol for FCGR3B/CD16b antibody 30201-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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