Pikachurin Monoclonal antibody 66232-1-Ig proteintech
$149.00
In stock
SKU
66232-1-Ig
| Catalog Number | 66232-1-Ig |
| Synonyms | EGFLAM, 1C6D12, Agrin like protein, AGRINL, Agrin-like protein |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA |
| Applications | WB, IF/ICC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | human retina tissue, PC-3 cells |
| Tested Applications — Positive IF-P detected in | rat eye tissue |
| Tested Applications — Positive IF/ICC detected in | Y79 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | human retina tissue, PC-3 cells |
| Positive IF-P detected in | rat eye tissue |
| Positive IF/ICC detected in | Y79 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6362 Product name: Recombinant human EGFLAM protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-152 aa of BC033177 Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK Predict reactive species |
| Full Name | EGF-like, fibronectin type III and laminin G domains |
| Calculated Molecular Weight | 111 kDa |
| Observed Molecular Weight | 111 kDa, 55-60 kDa |
| GenBank Accession Number | BC033177 |
| Gene Symbol | Pikachurin |
| Gene ID (NCBI) | 133584 |
| RRID | AB_2881623 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q63HQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| WB protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| human | IHC Int Immunopharmacol EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma Authors - Kaishu Li View Article |
| Federico (Verified Customer) (02-18-2020) | The antibody works extremely well in western blot analyses using both recombinant protein fragments produced in HEK293 cells as well as the endogenous protein from Y79 medium. Very sensitive and specific. Applications: Western Blot, Primary Antibody Dilution: 2000x Cell Tissue Type: overexpressing protein samples from HEK293 cells, conditioned media from Y79 cells |
| Citations | 2 |
| Dilutions | WB : 1:500-1:2000 IF-P : 1:200-1:800 IF/ICC : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1C6D12 |
| Conjugation | Unconjugated |
Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524). Protocols Product Specific Protocols IF protocol for Pikachurin antibody 66232-1-Ig Download protocol WB protocol for Pikachurin antibody 66232-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Comprehensive multi-omics pan-cancer analysis revealed EGFLAM as a potential prognostic and immune infiltration-associated biomarker
Journal: BMC Cancer | Authors: Jian Yang | Species: human | Application: WB - EGFLAM exhibits oncogenic activity and shows promise as a prognostic biomarker and therapeutic target in glioblastoma
Journal: Int Immunopharmacol | Authors: Kaishu Li | Species: human | Application: IHC
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