ACOT9 Monoclonal antibody 66532-1-Ig proteintech
$149.00
In stock
SKU
66532-1-Ig
| Catalog Number | 66532-1-Ig |
| Synonyms | acyl CoA thioesterase 9, 3F10G5, ACATE2, ACOT 9, Acyl CoA thioester hydrolase 9 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG2b |
| Tested Applications — Positive WB detected in | HEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:20000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8884 Product name: Recombinant human ACOT9 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-212 aa of BC012573 Sequence: MRRAALRLCALGKGQLTPGRGLTQGPQNPKKQGIFHIHEVRDKLREIVGASTNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKG Predict reactive species |
| Full Name | acyl-CoA thioesterase 9 |
| Calculated Molecular Weight | 24 kDa, 49 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC012573 |
| Gene Symbol | ACOT9 |
| Gene ID (NCBI) | 23597 |
| RRID | AB_2881895 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y305 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ACOT9 antibody 66532-1-Ig | Download protocol |
| IHC protocol for ACOT9 antibody 66532-1-Ig | Download protocol |
| WB protocol for ACOT9 antibody 66532-1-Ig | Download protocol |
| human | WB BMC Gastroenterol Establishment of prognostic prediction model based on lipid metabolism related genes in esophageal squamous cell carcinoma by machine learning algorithms. Authors - Peng Wang KD Validated View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:20000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Clone Number | 3F10G5 |
| Conjugation | Unconjugated |
ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa. Protocols Product Specific Protocols IF protocol for ACOT9 antibody 66532-1-Ig Download protocol IHC protocol for ACOT9 antibody 66532-1-Ig Download protocol WB protocol for ACOT9 antibody 66532-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma
Journal: Adv Sci (Weinh) | Authors: Qifan Hu | Species: human | Application: WB - Establishment of prognostic prediction model based on lipid metabolism related genes in esophageal squamous cell carcinoma by machine learning algorithms.
Journal: BMC Gastroenterol | Authors: Peng Wang KD Validated | Species: human | Application: WB
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