ALCAM Monoclonal antibody 67768-1-Ig proteintech
$149.00
In stock
SKU
67768-1-Ig
| Catalog Number | 67768-1-Ig |
| Synonyms | 1F7D6, CD166, MEMD |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HT-1080 cells, U2OS cells, A549 cells, LNCaP cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human breast cancer tissue |
| Tested Applications — Positive IF-Fro detected in | mouse breast cancer |
| Tested Applications — Positive IF/ICC detected in | hTERT-RPE1 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | HT-1080 cells, U2OS cells, A549 cells, LNCaP cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF-Fro detected in | mouse breast cancer |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag16892 Product name: Recombinant human ALCAM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 28-400 aa of BC137097 Sequence: WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTIVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVE Predict reactive species |
| Full Name | activated leukocyte cell adhesion molecule |
| Calculated Molecular Weight | 583 aa, 65 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC137097 |
| Gene Symbol | ALCAM |
| Gene ID (NCBI) | 214 |
| RRID | AB_2918534 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13740 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| IHC protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| WB protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| human | WB Antiviral Res Harnessing host enhancers of SARS-CoV-2 entry as novel targets for antiviral therapy Authors - Nathalia Williams View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF-P : 1:200-1:800 IF-FRO : 1:200-1:800 IF/ICC : 1:250-1:1000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1F7D6 |
| Conjugation | Unconjugated |
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759). Protocols Product Specific Protocols IF protocol for ALCAM antibody 67768-1-Ig Download protocol IHC protocol for ALCAM antibody 67768-1-Ig Download protocol WB protocol for ALCAM antibody 67768-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Harnessing host enhancers of SARS-CoV-2 entry as novel targets for antiviral therapy
Journal: Antiviral Res | Authors: Nathalia Williams | Species: human | Application: WB
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