Anti-Mouse CD45R (B220) (RA3-6B2) 65139-1-Ig proteintech
$50.00
In stock
SKU
65139-1-Ig
| Catalog Number | 65139-1-Ig |
| Synonyms | B220, CD45R, Cd45, Ptprc, EC:3.1.3.48 |
| Host | Rat |
| Reactivity | mouse |
| Form | Liquid |
| Formulation | PBS, Azide |
| Applications | IHC, IF/ICC, IF-P, FC |
| Host / Isotype | Rat / IgG2a, kappa |
| Tested Applications — Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse spleen tissue |
| Tested Applications — Positive IF/ICC detected in | mouse splenocytes |
| Tested Applications — Positive FC detected in | mouse splenocytes |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF/ICC detected in | mouse splenocytes |
| Positive FC detected in | mouse splenocytes |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Abelson murine leukemia virus-induced pre-B tumor cells Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| GenBank Accession Number | BC028512 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 19264 |
| RRID | AB_2918426 |
| Conjugate | Unconjugated |
| Purification Method | Affinity purification |
| UNIPROT ID | P06800 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
| FC protocol for CD45R (B220) antibody 65139-1-Ig | Download protocol |
| IF protocol for CD45R (B220) antibody 65139-1-Ig | Download protocol |
| IHC protocol for CD45R (B220) antibody 65139-1-Ig | Download protocol |
| mouse | IF Thorac Cancer Experimental model of micronodular thymic neoplasm with lymphoid stroma Authors - Riko Niwa View Article |
| Citations | 2 |
| Dilutions | IHC : 1:1000-1:4000 IF-P : 1:200-1:800 IF/ICC : 1:250-1:1000 |
| Isotype | IgG2a, kappa |
| Clonality | Monoclonal |
| Clone Number | RA3-6B2 |
| Conjugation | Unconjugated |
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R. Protocols Product Specific Protocols FC protocol for CD45R (B220) antibody 65139-1-Ig Download protocol IF protocol for CD45R (B220) antibody 65139-1-Ig Download protocol IHC protocol for CD45R (B220) antibody 65139-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- CCR7 orchestrates the malignant progression of memory B cells in MYC-overexpressing lymphoma.
Journal: Cell Death Differ | Authors: Yuwei Deng | Species: mouse | Application: IHC - Experimental model of micronodular thymic neoplasm with lymphoid stroma
Journal: Thorac Cancer | Authors: Riko Niwa | Species: mouse | Application: IF
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