CCDC50 Monoclonal antibody 68369-1-Ig proteintech
$149.00
In stock
SKU
68369-1-Ig
| Catalog Number | 68369-1-Ig |
| Synonyms | C3orf6, Coiled-coil domain-containing protein 50, DFNA44, Protein Ymer, YMER |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Tested Applications — Positive FC (Intra) detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:20000-1:100000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive FC (Intra) detected in | A549 cells |
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag15889 Product name: Recombinant human CCDC50 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-306 aa of BC065004 Sequence: MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQAQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYTHFTNQQSSTRHFSKSESSHKGFHYKH Predict reactive species |
| Full Name | coiled-coil domain containing 50 |
| Calculated Molecular Weight | 482 aa, 56 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC065004 |
| Gene Symbol | CCDC50 |
| Gene ID (NCBI) | 152137 |
| RRID | AB_3085089 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8IVM0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for CCDC50 antibody 68369-1-Ig | Download protocol |
| WB protocol for CCDC50 antibody 68369-1-Ig | Download protocol |
| human | WB EMBO Rep RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation Authors - Guoxiu Li View Article |
| Citations | 1 |
| Dilutions | WB : 1:20000-1:100000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 2F12G7 |
| Conjugation | Unconjugated |
CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326) Protocols Product Specific Protocols FC protocol for CCDC50 antibody 68369-1-Ig Download protocol WB protocol for CCDC50 antibody 68369-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation
Journal: EMBO Rep | Authors: Guoxiu Li | Species: human | Application: WB
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