GART Polyclonal antibody 13659-1-AP proteintech
$149.00
In stock
SKU
13659-1-AP
| Catalog Number | 13659-1-AP |
| Synonyms | 5'-phosphoribosylglycinamide transformylase, AIR synthase, AIRS, GAR transformylase, GARS |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, L02 cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, L02 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4555 Product name: Recombinant human GART protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC038958 Sequence: MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFVVVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSADFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVAPMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLTKNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLD Predict reactive species |
| Full Name | phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase |
| Calculated Molecular Weight | 1010 aa, 108 kDa |
| Observed Molecular Weight | 108 kDa, 46 kDa |
| GenBank Accession Number | BC038958 |
| Gene Symbol | GART |
| Gene ID (NCBI) | 2618 |
| RRID | AB_2107937 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22102 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GART antibody 13659-1-AP | Download protocol |
| IHC protocol for GART antibody 13659-1-AP | Download protocol |
| IP protocol for GART antibody 13659-1-AP | Download protocol |
| WB protocol for GART antibody 13659-1-AP | Download protocol |
| human | WB Cell Rep Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase. Authors - Natasha Emmanuel View Article |
| mouse | WB Nat Commun Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway. Authors - Mona Hoseini Soflaee View Article |
| Hua (Verified Customer) (08-26-2019) | Works well. Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: HepG2, mouse liver, mouse lung, mouse hippocampus GART Antibody Western Blot, validation (1:500 dilution) in HepG2, mouse liver, mouse lung, mouse hippocampus (Cat no:13659-1-AP) |
| Citations | 20 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
GART is a trifunctional polypeptide which has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity that is required for de novo purine biosynthesis. GART has 2 isoforms with molecular mass of 108 kDa and 46 kDa due to alternative splicing. Protocols Product Specific Protocols IF protocol for GART antibody 13659-1-AP Download protocol IHC protocol for GART antibody 13659-1-AP Download protocol IP protocol for GART antibody 13659-1-AP Download protocol WB protocol for GART antibody 13659-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.
Journal: Science | Authors: Issam Ben-Sahra - ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.
Journal: Mol Cell | Authors: Eunus S Ali | Species: human | Application: WB - LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma
Journal: Adv Sci (Weinh) | Authors: Yuwei Zhang | Species: human | Application: WB,IHC - Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.
Journal: Nat Commun | Authors: Mona Hoseini Soflaee | Species: mouse | Application: WB - UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.
Journal: EMBO J | Authors: Xing Feng | Species: human | Application: WB - Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.
Journal: Cell Rep | Authors: Natasha Emmanuel | Species: human | Application: WB - Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle.
Journal: Mol Cell | Authors: Mushtaq A. Nengroo - ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.
Journal: Mol Cell | Authors: Eunus S Ali | Species: human | Application: WB - GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer
Journal: Adv Sci (Weinh) | Authors: Chao Tang | Species: mouse,human | Application: WB,IHC,CoIP - LEDGF Binds H3R17me2a Promoting De Novo Nucleotide Biosynthesis in SETD2 Mutant Clear Cell Renal Cell Carcinoma
Journal: Adv Sci (Weinh) | Authors: Yuwei Zhang | Species: human | Application: WB,IHC - The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation
Journal: Oncogene | Authors: Nannan Sha | Species: human | Application: WB - UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.
Journal: EMBO J | Authors: Xing Feng | Species: human | Application: WB - Purine Nucleotide Availability Regulates mTORC1 Activity through the Rheb GTPase.
Journal: Cell Rep | Authors: Natasha Emmanuel | Species: human | Application: WB - Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota
Journal: Eur J Pharmacol | Authors: Wenwen Tan | Application: WB - GART promotes multiple myeloma malignancy via tumor stemness mediated by activating the HSP90α/CDK6/β-catenin axis
Journal: Eur J Pharmacol | Authors: Jinjun Qian | Species: human | Application: WB - Mechanistic insights into metformin's anti-hyperuricemic effect: Targeting PPP/DNPB/XOD-mediated purine pathway, purinosome assembly, and gut microbiota homostasis in rats.
Journal: Chem Biol Interact | Authors: Qiuxia Yu | Species: rat | Application: WB,IF - Prolonged Fructose Overconsumption Activates Original Biosynthetic and Metabolic Pathways of Endogenous Purine in Rats
Journal: Mol Nutr Food Res | Authors: Qiuxia Yu | Species: rat | Application: WB,IF - Succinate dehydrogenase activity supports de novo purine synthesis
Journal: bioRxiv | Authors: Mushtaq A Nengroo | Species: human | Application: WB - Succinate dehydrogenase activity supports de novo purine synthesis
Journal: bioRxiv | Authors: Mushtaq A Nengroo | Species: human | Application: WB - De novo purine synthesis reprograms the macrophage inflammatory response and the immune response in sepsis.
Journal: Res Sq | Authors: György Haskó | Species: mouse | Application: WB - Nanoplastics exacerbate DSS-induced colitis via histone lactate axis.
Journal: Ecotoxicol Environ Saf | Authors: Shan Tian
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
