GATA5 Polyclonal antibody 55433-1-AP proteintech
$149.00
In stock
SKU
55433-1-AP
| Catalog Number | 55433-1-AP |
| Synonyms | Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:200-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | GATA binding protein 5 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | NM_080473 |
| Gene Symbol | GATA5 |
| Gene ID (NCBI) | 140628 |
| RRID | AB_2881330 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GATA5 antibody 55433-1-AP | Download protocol |
| IHC protocol for GATA5 antibody 55433-1-AP | Download protocol |
| WB protocol for GATA5 antibody 55433-1-AP | Download protocol |
| human | WB Nat Commun Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy Authors - Wenjing Bai View Article |
| human,mouse | WB JACC Basic Transl Sci Disruption of Notch1 and Gata5 in Mice Leads to Congenital Aortic Valve Disease Authors - Jun Yasuhara View Article |
| mouse | WB Int J Biol Sci Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice Authors - Qianqian Feng View Article |
| Kamal (Verified Customer) (01-26-2024) | Works well when diluted in 1X TBST. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Mouse liver tissue |
| Citations | 5 |
| Dilutions | WB : 1:200-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.
Journal: Cancers (Basel) | Authors: Qinghua Wang | Species: human | Application: IP - Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways.
Journal: Biochem Biophys Res Commun | Authors: Anmin Ren | Species: human,mouse | Application: WB,IF,IP - Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy
Journal: Nat Commun | Authors: Wenjing Bai | Species: human | Application: WB - Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice
Journal: Int J Biol Sci | Authors: Qianqian Feng | Species: mouse | Application: WB - Disruption of Notch1 and Gata5 in Mice Leads to Congenital Aortic Valve Disease
Journal: JACC Basic Transl Sci | Authors: Jun Yasuhara | Species: human,mouse | Application: WB
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