GBF1 Polyclonal antibody 25183-1-AP proteintech
$149.00
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SKU
25183-1-AP
| Catalog Number | 25183-1-AP |
| Synonyms | ARF1GEF, BFA resistant GEF 1, GBF1, KIAA0248 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag16579 Product name: Recombinant human GBF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1506-1855 aa of BC117681 Sequence: ASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQVRMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRASTLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDTAEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAHLTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGPPPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN Predict reactive species |
| Full Name | golgi-specific brefeldin A resistant guanine nucleotide exchange factor 1 |
| Calculated Molecular Weight | 1859 aa, 206 kDa |
| Observed Molecular Weight | 206 kDa |
| GenBank Accession Number | BC117681 |
| Gene Symbol | GBF1 |
| Gene ID (NCBI) | 8729 |
| RRID | AB_2879948 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92538 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GBF1 antibody 25183-1-AP | Download protocol |
| IHC protocol for GBF1 antibody 25183-1-AP | Download protocol |
| WB protocol for GBF1 antibody 25183-1-AP | Download protocol |
| human | IF J Virol Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1 Authors - Junki Hirano View Article |
| Citations | 4 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- TBC1D23 mediates Golgi-specific LKB1 signaling
Journal: Nat Commun | Authors: Yingfeng Tu | Species: human | Application: WB - Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
Journal: PLoS Pathog | Authors: Tao Wang | Species: human | Application: WB - The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.
Journal: Sci Rep | Authors: Xiaobo Lei KD Validated | Species: human | Application: WB - Enterovirus 3A protein disrupts endoplasmic reticulum homeostasis through interaction with GBF1
Journal: J Virol | Authors: Junki Hirano | Species: human | Application: IF
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