GCK Polyclonal antibody 15629-1-AP proteintech
$149.00
In stock
SKU
15629-1-AP
| Catalog Number | 15629-1-AP |
| Synonyms | HK4, HK IV, Hexokinase-D, Hexokinase-4, Glucokinase |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse liver tissue, rat liver tissue, pig liver tissue |
| Tested Applications — Positive IP detected in | mouse liver tissue |
| Tested Applications — Positive IHC detected in | human liver cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse liver tissue, rat liver tissue, pig liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7904 Product name: Recombinant human GCK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 157-465 aa of BC001890 Sequence: EDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ Predict reactive species |
| Full Name | glucokinase (hexokinase 4) |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC001890 |
| Gene Symbol | GCK |
| Gene ID (NCBI) | 2645 |
| RRID | AB_2878159 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35557 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for GCK antibody 15629-1-AP | Download protocol |
| IP protocol for GCK antibody 15629-1-AP | Download protocol |
| WB protocol for GCK antibody 15629-1-AP | Download protocol |
| mouse | WB Mol Cell Endocrinol Improved lipogenesis gene expression in liver is associated with elevated plasma angiotensin 1-7 after AT1 receptor blockade in insulin-resistant OLETF rats Authors - Jose A Godoy-Lugo View Article |
| Citations | 3 |
| Dilutions | WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa. The westernblotting results of human cells we tested are not well, therefore, we do not recommend it to customers who would like to do WB involved in human. Protocols Product Specific Protocols IHC protocol for GCK antibody 15629-1-AP Download protocol IP protocol for GCK antibody 15629-1-AP Download protocol WB protocol for GCK antibody 15629-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Using recombinase-mediated cassette exchange to engineer MIN6 insulin-secreting cells based on a newly identified safe harbor locus.
Journal: J Diabetes Investig | Authors: Asami Furukawa KD Validated | Species: mouse | Application: WB - Compromised chronic efficacy of a glucokinase activator AZD1656 in mouse models for common human GCKR variants
Journal: Biochem Pharmacol | Authors: Brian E Ford | Species: mouse | Application: WB,IHC - Improved lipogenesis gene expression in liver is associated with elevated plasma angiotensin 1-7 after AT1 receptor blockade in insulin-resistant OLETF rats
Journal: Mol Cell Endocrinol | Authors: Jose A Godoy-Lugo | Species: mouse | Application: WB
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