GCSH Recombinant monoclonal antibody 83012-5-RR proteintech
$149.00
In stock
SKU
83012-5-RR
| Catalog Number | 83012-5-RR |
| Synonyms | GCE, GCSH, NKH |
| Host | Rabbit |
| Reactivity | Human |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | Human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag10174 Product name: Recombinant human GCSH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC000790 Sequence: MALRVVRSVRALLCTLRAVPLPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE Predict reactive species |
| Full Name | glycine cleavage system protein H (aminomethyl carrier) |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | ~15 kDa |
| GenBank Accession Number | BC000790 |
| Gene Symbol | GCSH |
| Gene ID (NCBI) | 2653 |
| RRID | AB_3670756 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P23434 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for GCSH antibody 83012-5-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230505A3 |
| Conjugation | Unconjugated |
GCSH(Glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the mitochondrial inner membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. The lipoylation of H-protein occurs in mitochondria which probably contain an activated form of lipoic acid as well as other components required for the transfer of lipoic acid to the protein(PMID:2211640). Defects in GCSH are a cause of non-ketotic hyperglycinemia (NKH). Protocols Product Specific Protocols WB protocol for GCSH antibody 83012-5-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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