CUL2 Monoclonal antibody 67175-1-Ig proteintech
$149.00
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SKU
67175-1-Ig
| Catalog Number | 67175-1-Ig |
| Synonyms | CUL 2, CUL-2, cullin 2, Cullin-2 |
| Host | Mouse |
| Reactivity | human, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, MCF-7 cells, PC-3 cells, Raji cells, SW 1990 cells, TF-1 cells, K-562 cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | A431 cells, HEK-293 cells, HeLa cells, HSC-T6 cells, MCF-7 cells, PC-3 cells, Raji cells, SW 1990 cells, TF-1 cells, K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag28654 Product name: Recombinant human CUL2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 341-745 aa of BC009591 Sequence: ESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA Predict reactive species |
| Full Name | cullin 2 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC009591 |
| Gene Symbol | CUL2 |
| Gene ID (NCBI) | 8453 |
| RRID | AB_2882471 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13617 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| IHC protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| WB protocol for CUL2 antibody 67175-1-Ig | Download protocol |
| human | WB,IP,IF FEBS J TRAF2 associates with cullin neddylation complex assembly Authors - Tiantian Wang View Article |
| Citations | 2 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1A6G9 |
| Conjugation | Unconjugated |
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. Protocols Product Specific Protocols IF protocol for CUL2 antibody 67175-1-Ig Download protocol IHC protocol for CUL2 antibody 67175-1-Ig Download protocol WB protocol for CUL2 antibody 67175-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Orlistat targets NEDD8 conjugating enzyme UBC12 for cancer therapy.
Journal: Pharmacol Res | Authors: Mengzhen Shen | Species: human | Application: WB - TRAF2 associates with cullin neddylation complex assembly
Journal: FEBS J | Authors: Tiantian Wang | Species: human | Application: WB,IP,IF
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