Cyclin D3 Monoclonal antibody 66357-1-Ig proteintech
$149.00
In stock
SKU
66357-1-Ig
| Catalog Number | 66357-1-Ig |
| Synonyms | CCND3, 1F2C6, Cyclin D, G1/S specific cyclin D3, G1/S-specific cyclin-D3 |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | Raji cells, Jurkat cells, HL-60 cells, HEK-293 cells, K-562 cells |
| Tested Applications — Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | Raji cells, Jurkat cells, HL-60 cells, HEK-293 cells, K-562 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag22825 Product name: Recombinant human Cyclin D3 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-292 aa of BC011616 Sequence: MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQREIKPHMRKMLAYWMLEVCEEQRCEEEVFPLAMNYLDRYLSCVPTRKAQLQLLGAVCMLLASKLRETTPLTIEKLCIYTDHAVSPRQLRDWEVLVLGKLKWDLAAVIAHDFLAFILHRLSLPRDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL Predict reactive species |
| Full Name | cyclin D3 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC011616 |
| Gene Symbol | Cyclin D3 |
| Gene ID (NCBI) | 896 |
| ENSEMBL Gene ID | ENSG00000112576 |
| RRID | AB_2881736 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P30281 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Cyclin D3 antibody 66357-1-Ig | Download protocol |
| WB protocol for Cyclin D3 antibody 66357-1-Ig | Download protocol |
| human | WB Heliyon PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by ubiquitinating CTMP and p21 Authors - Kai Sun View Article |
| Janardhan (Verified Customer) (08-22-2020) | Antibody works well for western blot Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Medulloblastoma |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:8000 IHC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1F2C6 |
| Conjugation | Unconjugated |
Cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activitiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Protocols Product Specific Protocols IHC protocol for Cyclin D3 antibody 66357-1-Ig Download protocol WB protocol for Cyclin D3 antibody 66357-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Interferon-stimulated gene screening identifies CCND3 as a host restriction factor against emerging high-pathogenic bandaviruses
Journal: Nat Commun | Authors: Zhao Xu | Species: human | Application: WB,IP,IF - PRAME promotes proliferation of multiple myeloma cells through CTMP/Akt/p21/CCND3 axis by ubiquitinating CTMP and p21
Journal: Heliyon | Authors: Kai Sun | Species: human | Application: WB
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