GEMIN8 Monoclonal antibody 67871-1-Ig proteintech
$149.00
In stock
SKU
67871-1-Ig
| Catalog Number | 67871-1-Ig |
| Synonyms | 1A6B4, FAM51A1, Gem-associated protein 8, Gemin 8, Gemin-8 |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | NIH/3T3 cells, HeLa cells |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | NIH/3T3 cells, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6774 Product name: Recombinant human GEMIN8 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-242 aa of BC003607 Sequence: MAAVKASTSKATRPWYSHPVYARYWQHYHQAMAWMQSHHNAYRKAVESCFNLPWYLPSALLPQSSYDNEAAYPQSFYDHHVAWQDYPCSSSHFRRSGQHPRYSSRIQASTKEDQALSKEEEMETESDAEVECDLSNMEITEELRQYFAETERHREERRRQQQLDAERLDSYVNADHDLYCNTRRSVEAPTERPGERRQAEMKRLYGDSAAKIQAMEAAVQLSFDKHCDRKQPKYWPVIPLKF Predict reactive species |
| Full Name | gem (nuclear organelle) associated protein 8 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC003607 |
| Gene Symbol | GEMIN8 |
| Gene ID (NCBI) | 54960 |
| RRID | AB_2918629 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NWZ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GEMIN8 antibody 67871-1-Ig | Download protocol |
| IHC protocol for GEMIN8 antibody 67871-1-Ig | Download protocol |
| WB protocol for GEMIN8 antibody 67871-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1A6B4 |
| Conjugation | Unconjugated |
GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only. Protocols Product Specific Protocols IF protocol for GEMIN8 antibody 67871-1-Ig Download protocol IHC protocol for GEMIN8 antibody 67871-1-Ig Download protocol WB protocol for GEMIN8 antibody 67871-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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