Cytokeratin 5/6 Monoclonal antibody 68295-1-Ig proteintech
$149.00
In stock
SKU
68295-1-Ig
| Catalog Number | 68295-1-Ig |
| Synonyms | KRT6B, 1F9E1, CK 6B, CK5/6, CK6B |
| Host | Mouse |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue, human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human cervical cancer tissue |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HaCaT cells, A431 cells, human saliva, rat skin tissue, mouse skin tissue |
| Positive IHC detected in | human tonsillitis tissue, human cervical cancer tissue, human lung cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cervical cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag11288 Product name: Recombinant human KRT6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 214-564 aa of BC034535 Sequence: FEQYINNLRRQLDNIVGERGRLDSELRNMQDLVEDLKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQITAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRATGGGLSSVGGGSSTIKYTTTSSSSRKSYKH Predict reactive species |
| Full Name | keratin 6B |
| Calculated Molecular Weight | 564 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC034535 |
| Gene Symbol | Cytokeratin 6B |
| Gene ID (NCBI) | 3854 |
| RRID | AB_2935375 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P04259 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| WB protocol for Cytokeratin 5/6 antibody 68295-1-Ig | Download protocol |
| human | IF Breast Cancer Res CD19(+)Ki67(+)B cells regulated by NAMPT as key modulators in triple-negative breast cancer with brain metastasis. Authors - Qiyi Zhou View Article |
| monkey | IHC J Comp Pathol Myoepithelial-rich pleomorphic adenoma of the soft palate in a rhesus monkey (Macaca mulatta) Authors - Matthew F Starost View Article |
| Citations | 6 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:5000-1:20000 IF-P : 1:200-1:800 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1F9E1 |
| Conjugation | Unconjugated |
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. This antibody can react with Cytokeratin 5 and Cytokeratin 6. Protocols Product Specific Protocols IF protocol for Cytokeratin 5/6 antibody 68295-1-Ig Download protocol IHC protocol for Cytokeratin 5/6 antibody 68295-1-Ig Download protocol WB protocol for Cytokeratin 5/6 antibody 68295-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Genetically engineered Salmonella, SGN1, as a tumor microenvironment-targeting modality capable of methionine reduction for oral squamous cell carcinoma.
Journal: J Control Release | Authors: Ping Mao | Species: human | Application: IHC - TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma
Journal: Cancer Res | Authors: Zhao Ding | Species: human | Application: IHC - Protocol for the establishment and characterization of lung tumoroids from surgical resection without the use of Matrigel
Journal: STAR Protoc | Authors: Federico Donà - Myoepithelial-rich pleomorphic adenoma of the soft palate in a rhesus monkey (Macaca mulatta)
Journal: J Comp Pathol | Authors: Matthew F Starost | Species: monkey | Application: IHC - Molecular Switching from Morule to Squamous Metaplasia via p40 and the EBP50/S100A4/Myosin 9 Axis in Endometrial Carcinoma.
Journal: Am J Pathol | Authors: Ako Yokoi | Species: human | Application: IHC - CD19(+)Ki67(+)B cells regulated by NAMPT as key modulators in triple-negative breast cancer with brain metastasis.
Journal: Breast Cancer Res | Authors: Qiyi Zhou | Species: human | Application: IF
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