ChromoTek GFP-Trap® Magnetic Particles M-270 gtd proteintech
$352.00
In stock
SKU
gtd
| Synonyms | GFP Trap Magnetic Particles M-270, GFP-Trap Magnetic Particles M-270, gtd, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400, gfp, GFP-Trap® Magnetic Particles M-270, gtd-10, gtd-100, gtd-20, gtd-200, gtd-400 |
| Host | Alpaca |
| Formulation | United States |
| Applications | IP, CoIP, ChIP, RIP |
| Host / Isotype | IP, CoIP, ChIP, RIP |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 2-2.8 μg of recombinant GFP per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270, size: 2.8 µm high throughput-compatible |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 10 mM DTT, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 0.2 % SDS, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | Dissociation constant K D of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827592 |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg:20rxns=500ul slurry) |
| IP | Neuron Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons. Authors - Juliet Goldsmith View Article |
| CoIP | Nat Commun Ustilago maydis disrupts carbohydrate signaling networks to induce hypertrophy in host cells. Authors - Yoon Joo Lee View Article |
| FH matthieu (Verified Customer) (04-30-2024) | Best way to trap GFP derivatives for immunoprecipitation (tested on S65T GFP, EGFP , yeGFP). Very efficient with no or low background. Applications: Immunoprecipitation Cell Tissue Type: Yeast |
| FH Anastasija (Verified Customer) (02-14-2022) | I used the GFP-trap dynabeads to perform co-IP of overexpressed GFP-tagged proteins in HEK293T cells. The pulldown was highly enriched in the bait and expected interactors, with low background. I am very satisfied with the performance of the product and will use it again. Applications: Immunoprecipitation Cell Tissue Type: HEK293T |
Description
Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic particles. GFP-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP. • IP and Co-IP of GFP-tagged proteins without size limitation • Magnetic separation with easy and efficient washing of GFP-Trap Magnetic Particles M-270 • Automation and high throughput applications • No heavy & light antibody chains, short incubation (5-30 min) • Extraordinary binding, also under harsh conditions • Very high affinity (KD=1 pM) to bind even low abundant proteins
Publications