ChromoTek anti-GFP recombinant VHH, unconjugated gt proteintech
$383.00
In stock
SKU
gt
| Synonyms | GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb) |
| Host | Alpaca |
| Molecular Weight | 13.9 kDa |
| Formulation | United States |
| Applications | Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction |
| Host / Isotype | Conjugation |
| Specificity/Target | CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine |
| Conjugate | Unconjugated |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | Dissociation constant K D of 1 pM |
| RRID | AB_2631361 |
| Storage Buffer | 25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze. |
| Size | 250 µg |
| Background | Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This Nanobody is an alpaca recombinant VHH raised against GFP, reactive to a variety of GFP derivates, including CFP, eGFP, yeast eGFP, Clover, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, YPET, Citrine and eCitrine and more. For a full list of specificities see: Fluorescent_Specificity_by_Nano-Trap_190222.pdf (ptglab.com) |
| IF | Nat Protoc Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions. Authors - Sumit K Chaturvedi View Article |
Description
Alpaca anti-GFP VHH, purified recombinant binding protein for extraordinary stable & reliable binding • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate • Fulfills highest requirements for antibody validation • Structure and function are characterized
Publications
- DART.2: bidirectional synaptic pharmacology with thousandfold cellular specificity
Journal: Nat Methods | Authors: Brenda C Shields | Application: Nat Methods DART.2: bidirectional synaptic pharmacology with thousandfold cellular specificity Authors - Brenda C Shields View Article - TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity.
Journal: Nature | Authors: Manoël Prouteau | Species: IF | Application: Nature TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity. Authors - Manoël Prouteau View Article - Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells.
Journal: Cell | Authors: Jieru Li | Species: IF | Application: Cell Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells. Authors - Jieru Li View Article - Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Journal: Nat Methods | Authors: Yongdeng Zhang | Application: Nat Methods Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging. Authors - Yongdeng Zhang View Article - Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.
Journal: Nat Protoc | Authors: Sumit K Chaturvedi | Species: IF | Application: Nat Protoc Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions. Authors - Sumit K Chaturvedi View Article - Nanobody-based products as research and diagnostic tools.
Journal: Trends Biotechnol | Authors: Thomas De Meyer | Application: Trends Biotechnol Nanobody-based products as research and diagnostic tools. Authors - Thomas De Meyer View Article - Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.
Journal: Nat Protoc | Authors: Sumit K Chaturvedi | Application: IF - Exploring Cell Surface-Nanopillar Interactions with 3D Super-Resolution Microscopy
Journal: ACS Nano | Authors: Anish R Roy | Species: human,mouse | Application: IF - Nanobody-based products as research and diagnostic tools.
Journal: Trends Biotechnol | Authors: Thomas De Meyer - Membrane phase separation drives responsive assembly of receptor signaling domains
Journal: Nat Chem Biol | Authors: Sarah A Shelby - Versatile Detection of Cellular Protein via Fluorescence Anisotropy.
Journal: Adv Sci (Weinh) | Authors: Qing Tang - Litmus-Body: A Molecularly Targeted Sensor for Cell-Surface pH Measurements.
Journal: ACS Sens | Authors: Joe Chin-Hun Kuo | Application: IP - Multimerization and Retention of the Scavenger Receptor SR-B1 in the Plasma Membrane.
Journal: Dev Cell | Authors: Pedro E Marques | Application: IF - The cyclimids: Degron-inspired cereblon binders for targeted protein degradation
Journal: Cell Chem Biol | Authors: Saki Ichikawa - Nanobody-Based Biotemplating Enables Nanoscale-Precision Metallization of Cellular, Tissue, and Plant Architectures.
Journal: ACS Appl Mater Interfaces | Authors: Hoeyun Jung - A family of C. elegans VASA homologs control Argonaute pathway specificity and promote transgenerational silencing
Journal: Cell Rep | Authors: Siyuan Dai - Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes.
Journal: Cell Rep | Authors: Sivakami Mylvaganam | Application: WB - AT Hook-Like 10 phosphorylation determines ribosomal RNA processing 6-like 1 (RRP6L1) chromatin association and growth suppression during water stress
Journal: Plant Cell Environ | Authors: Min May Wong | Species: yeast | Application: ChIP - SCRIB controls apical contractility during epithelial differentiation
Journal: J Cell Biol | Authors: Batiste Boëda | Species: human - Regulation of cell dynamics by rapid integrin transport through the biosynthetic pathway.
Journal: J Cell Biol | Authors: Martina Lerche - Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.
Journal: Int J Mol Sci | Authors: Marius Terfrüchte | Application: ELISA - Crosstalk between invadopodia and the extracellular matrix.
Journal: Eur J Cell Biol | Authors: Shinji Iizuka | Application: IF - mRNA localization is linked to translation regulation in the Caenorhabditis elegans germ lineage.
Journal: Development | Authors: Dylan M Parker | Application: IF - An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules
Journal: J Cell Sci | Authors: Fabienne De Graeve | Application: IP - An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules
Journal: J Cell Sci | Authors: Fabienne De Graeve | Application: IP - A simple method for GFP- and RFP-based dual color single-molecule localization microscopy.
Journal: ACS Chem Biol | Authors: Evgenia Platonova | Application: IF - Evidence for endogenous exchange of cytoplasmic material between a subset of cone and rod photoreceptors within the adult mammalian retina via direct cell-cell connections.
Journal: Exp Eye Res | Authors: Patrick Heisterkamp | Application: IF - Ubiquitin orchestrates proteasome dynamics between proliferation and quiescence in yeast.
Journal: Mol Biol Cell | Authors: Zhu Chao Gu | Application: IF - Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa.
Journal: Fungal Genet Biol | Authors: Phillip Ringel - Quantitative localization microscopy: effects of photophysics and labeling stoichiometry.
Journal: PLoS One | Authors: Robert P J Nieuwenhuizen | Application: IF - Quantitative localization microscopy: effects of photophysics and labeling stoichiometry.
Journal: PLoS One | Authors: Robert P J Nieuwenhuizen | Application: IF - Dual color single particle tracking via nanobodies.
Journal: Methods Appl Fluoresc | Authors: David Albrecht | Application: IF - Improved Methods for Single-Molecule Fluorescence In Situ Hybridization and Immunofluorescence in Caenorhabditis elegans Embryos.
Journal: Curr Protoc | Authors: Dylan M Parker | Application: IF - The Antitumor Effect of Single-domain Antibodies Directed Towards Membrane-associated Catalase and Superoxide Dismutase.
Journal: Anticancer Res | Authors: Georg Bauer - Imaging the assembly, structure, and function of the nuclear pore inside cells.
Journal: Methods Cell Biol | Authors: Shotaro Otsuka | Application: IP - Localization microscopy in yeast.
Journal: Methods Cell Biol | Authors: Markus Mund - Protocol for the comprehensive biochemical and cellular profiling of small-molecule degraders using CoraFluor TR-FRET technology
Journal: STAR Protoc | Authors: N Connor Payne - Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes.
Journal: Bio Protoc | Authors: Ammarah Tariq - RNAi-Based Screening for the Identification of Specific Substrate-Deubiquitinase Pairs
Journal: Methods Mol Biol | Authors: Nagore Elu
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