Dynamitin (1-401 AA) Monoclonal antibody 60257-1-Ig proteintech
$149.00
In stock
SKU
60257-1-Ig
| Catalog Number | 60257-1-Ig |
| Synonyms | DCTN2, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin |
| Host | Mouse |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF, IHC, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Mouse / IgG3 |
| Tested Applications — Positive WB detected in | human brain tissue |
| Tested Applications — Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1902 Product name: Recombinant human dynactin-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-401 aa of BC014083 Sequence: MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK Predict reactive species |
| Full Name | dynactin 2 (p50) |
| Calculated Molecular Weight | 401 aa, 44 kDa |
| Observed Molecular Weight | 52+150 kDa |
| GenBank Accession Number | BC014083 |
| Gene Symbol | Dynamitin |
| Gene ID (NCBI) | 10540 |
| RRID | AB_2881378 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13561 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| WB protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:20-1:200 |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Clone Number | 2B9A5 |
| Conjugation | Unconjugated |
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin. Protocols Product Specific Protocols IHC protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig Download protocol WB protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster.
Journal: Dev Biol | Authors: Chun-Hong Wu | Application: IF
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