GLDC Recombinant monoclonal antibody 86378-1-RR proteintech
$149.00
In stock
SKU
86378-1-RR
| Catalog Number | 86378-1-RR |
| Synonyms | aminomethyl-transferring, EC:1.4.4.2, GCE, GCSP, Glycine dehydrogenase |
| Host | Rabbit |
| Reactivity | human, mouse, rat, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, Vero cells, mouse liver tissue, rat liver tissue |
| Tested Applications — Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | HepG2 cells, Vero cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat, monkey |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag20410 Product name: Recombinant human GLDC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 675-1020 aa of BC111995 Sequence: NIDAVHLKAMVDKHKENLAAIMITYPSTNGVFEENISDVCDLIHQHGGQVYLDGANMNAQVGICRPGDFGSDVSHLNLHKTFCIPHGGGGPGMGPIGVKKHLAPFLPNHPVISLKRNEDACPVGTVSAAPWGSSSILPISWAYIKMMGGKGLKQATETAILNANYMAKRLETHYRILFRGARGYVGHEFILDTRPFKKSANIEAVDVAKRLQDYGFHAPTMSWPVAGTLMVEPTESEDKAELDRFCDAMISIRQEIADIEEGRIDPRVNPLKMSPHSLTCVTSSHWDRPYSREVAAFPLPFVKPENKFWPTIARIDDIYGDQHLVCTCPPMEVYESPFSEQKRASS Predict reactive species |
| Full Name | glycine dehydrogenase (decarboxylating) |
| Calculated Molecular Weight | 1020 aa, 113 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC111995 |
| Gene Symbol | GLDC |
| Gene ID (NCBI) | 2731 |
| RRID | AB_3744850 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P23378 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for GLDC antibody 86378-1-RR | Download protocol |
| WB protocol for GLDC antibody 86378-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250916A5 |
| Conjugation | Unconjugated |
GLDC(Glycine dehydrogenase [decarboxylating], mitochondrial) is also named as GCSP and belongs to the GcvP family. It induces dramatic changes in glycolysis and glycine/serine metabolism that leads to changes in pyrimidine metabolism to regulate cancer cell proliferation(PMID:22225612). Protocols Product Specific Protocols IHC protocol for GLDC antibody 86378-1-RR Download protocol WB protocol for GLDC antibody 86378-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols