| Catalog Number | 16508-1-AP |
| Synonyms | Glioma pathogenesis-related protein 2, GLI pathogenesis related 2, GAPR-1, GAPR1, GAPR 1 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human milk |
| Tested Applications — Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | human milk |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9725 Product name: Recombinant human GLIPR2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-90 aa of BC017918 Sequence: MGKSASKQFHNEVLKAHNEYRQKHGVPPLKLCKNLNREAQQYSEALASTRILKHSPESSRGQCGENLAWASYDQTGKEVADRWYSEVHFM Predict reactive species |
| Full Name | GLI pathogenesis-related 2 |
| Calculated Molecular Weight | 153aa,17 kDa; 90aa,10 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC017918 |
| Gene Symbol | GLIPR2 |
| Gene ID (NCBI) | 152007 |
| RRID | AB_3669246 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4G4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GLIPR2 antibody 16508-1-AP | Download protocol |
| IHC protocol for GLIPR2 antibody 16508-1-AP | Download protocol |
| WB protocol for GLIPR2 antibody 16508-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Golgi-associated plant pathogenesis-related protein 1 (GLIPR2, also known as C9orf19 and GAPR1) is a multifunctional protein with dual engagement in normal cellular processes and the intricacies of cancer biology. GLIPR2 has also been associated with a spectrum of cellular functions encompassing the regulation of autophagy (PMID: 12137952; 18824526). It has been reported that GLIPR2 is a newly identified negative regulator of autophagy that inhibits the class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1) complex activity (PMID: 33222586). Protocols Product Specific Protocols IF protocol for GLIPR2 antibody 16508-1-AP Download protocol IHC protocol for GLIPR2 antibody 16508-1-AP Download protocol WB protocol for GLIPR2 antibody 16508-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols