FOXN1 Monoclonal antibody 66337-1-Ig proteintech
$149.00
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SKU
66337-1-Ig
| Catalog Number | 66337-1-Ig |
| Synonyms | FKHL20, forkhead box N1, Forkhead box protein N1, RONU, WHN |
| Host | Mouse |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, FC (Intra), ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | Raji cells, Jurkat cells |
| Tested Applications — Positive IHC detected in | mouse thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive FC (Intra) detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | Raji cells, Jurkat cells |
| Positive IHC detected in | mouse thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag18207 Product name: Recombinant human FOXN1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 261-648 aa of BC146539 Sequence: STDGHQPLFPKPIYSYSILIFMALKNSKTGSLPVSEIYNFMTEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVENKSGSSSRKGCLWALNPAKIDKMQEELQKWKRKDPIAVRKSMAKPEELDSLIGDKREKLGSPLLGCPPPGLSGSGPIRPLAPPAGLSPPLHSLHPAPGPIPGKNPLQDLLMGHTPSCYGQTYLHLSPGLAPPGPPQPLFPQPDGHLELRAQPGTPQDSPLPAHTPPSHSAKLLAEPSPARTMHDTLLPDGDLGTDLDAINPSLTDFDFQGNLWEQLKDDSLALDPLVLVTSSPTSSSMPPPQPPPHCFPPGPCLTETGSGAGDLAAPGSGGSGALGDLHLTTLYSAFMELEPTPPTAPAGPSVYLSPSSKPVALA Predict reactive species |
| Full Name | forkhead box N1 |
| Calculated Molecular Weight | 648 aa, 69 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC146539 |
| Gene Symbol | FOXN1 |
| Gene ID (NCBI) | 8456 |
| RRID | AB_2881717 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | O15353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
| WB protocol for FOXN1 antibody 66337-1-Ig | Download protocol |
| human | WB Nat Commun Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA Authors - Ya Nan Zhu View Article |
| Citations | 1 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:250-1:1000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1D8B12 |
| Conjugation | Unconjugated |
FOXN1, also named as Forkhead box protein N1, is a 648 amino acid protein, which contains 1 fork-head DNA-binding domain and localizes in the nucleus. FOXN1 as transcriptional regulator regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. FOXN1 acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Protocols Product Specific Protocols IHC protocol for FOXN1 antibody 66337-1-Ig Download protocol WB protocol for FOXN1 antibody 66337-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Parental experiences orchestrate locust egg hatching synchrony by regulating nuclear export of precursor miRNA
Journal: Nat Commun | Authors: Ya Nan Zhu | Species: human | Application: WB
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