GOLIM4 Recombinant monoclonal antibody 84818-5-RR proteintech
$149.00
In stock
SKU
84818-5-RR
| Catalog Number | 84818-5-RR |
| Synonyms | 242323F4, GIMPC, GOLPH4, GPP130, P 138 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, HT-1080 cells, L02 cells, C2C12 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | U-2 OS, HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, HT-1080 cells, L02 cells, C2C12 cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | U-2 OS, HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag34962 Product name: Recombinant human GOLIM4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 350-500 aa of NM_014498 Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ Predict reactive species |
| Full Name | golgi integral membrane protein 4 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_014498 |
| Gene Symbol | GOLIM4 |
| Gene ID (NCBI) | 27333 |
| RRID | AB_3743759 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | O00461 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for GOLIM4 antibody 84818-5-RR | Download protocol |
| IF protocol for GOLIM4 antibody 84818-5-RR | Download protocol |
| WB protocol for GOLIM4 antibody 84818-5-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242323F4 |
| Conjugation | Unconjugated |
Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697). Protocols Product Specific Protocols FC protocol for GOLIM4 antibody 84818-5-RR Download protocol IF protocol for GOLIM4 antibody 84818-5-RR Download protocol WB protocol for GOLIM4 antibody 84818-5-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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