G3BP2 Recombinant monoclonal antibody 82080-6-RR proteintech
$149.00
In stock
SKU
82080-6-RR
| Catalog Number | 82080-6-RR |
| Synonyms | G3BP 2, 230275G3, G3BP-2, GAP SH3 domain binding protein 2, GAP SH3 domain-binding protein 2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, HEK-293 cells, A549 cells, Jurkat cells, MCF-7 cells, mouse cerebellum tissue |
| Tested Applications — Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, HEK-293 cells, A549 cells, Jurkat cells, MCF-7 cells, mouse cerebellum tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
| Positive FC (Intra) detected in | U2OS cells |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag9355 Product name: Recombinant human G3BP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 97-449 aa of BC011731 Sequence: LLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELKPQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGDMEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSEPVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRGPPPRGGMAQKLGSGRGTGQMEGRFTGQRR Predict reactive species |
| Full Name | GTPase activating protein (SH3 domain) binding protein 2 |
| Calculated Molecular Weight | 482aa,54 kDa; 449aa,51 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC011731 |
| Gene Symbol | G3BP2 |
| Gene ID (NCBI) | 9908 |
| RRID | AB_3670514 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UN86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for G3BP2 antibody 82080-6-RR | Download protocol |
| IF protocol for G3BP2 antibody 82080-6-RR | Download protocol |
| IHC protocol for G3BP2 antibody 82080-6-RR | Download protocol |
| WB protocol for G3BP2 antibody 82080-6-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:500-1:2000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 230275G3 |
| Conjugation | Unconjugated |
Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer. Protocols Product Specific Protocols FC protocol for G3BP2 antibody 82080-6-RR Download protocol IF protocol for G3BP2 antibody 82080-6-RR Download protocol IHC protocol for G3BP2 antibody 82080-6-RR Download protocol WB protocol for G3BP2 antibody 82080-6-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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