ChromoTek HA-Trap Magnetic Agarose atma proteintech
$289.00
In stock
SKU
atma
| Synonyms | Hemagglutinin tag, HA, HA-tag, HA-tag Trap Magnetic Agarose |
| Host | Alpaca |
| Formulation | United States |
| Applications | IP, Co-IP |
| Host / Isotype | IP, Co-IP |
| Specificity/Target | Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low. |
| Binding Capacity | 20 ug recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry |
| Conjugate | Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads) |
| Elution buffer | 2x SDS-sample buffer (Lammli) |
| Wash Buffer Compatibility | 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions. |
| Compatibility with mass spectrometry | The HA-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3094561 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
| CoIP | Plant Cell Maize COMPACT PLANT 3 regulates plant architecture and facilitates high-density planting Authors - Huangjun Sheng View Article |
| IP | Plant Physiol ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis Authors - Wan-Ni Wang View Article |
| FH Denzel (Verified Customer) (07-27-2026) | Highly specific and easy to use. The magnetic properties make the workflow fast and efficient, with the added benefit of no heavy and light antibody chain contamination. Applications: Western Blot,Immunoprecipitation |
| FH Shelly (Verified Customer) (05-01-2025) | worked better than other anti-HA beads that we tried Applications: Immunoprecipitation |
| FH Angelique (Verified Customer) (03-28-2025) | Easy to use. Works well in both IP and Co-IP Applications: Immunoprecipitation Cell Tissue Type: squamous epithelial cancer cell line |
| FH Matheus (Verified Customer) (03-12-2025) | 25uL of the beads were enough for me to get some protein bound to it and notice it worked. Despite the fact the protein bound, I could also notice I got extra bands, which I am still not sure whether they bound to my protein or the beads themselves. As I used the sample to test whether the protein was being expressed and was sufficient to load the beads for immunoprecipitation, I can see it worked fine. Applications: Immunoprecipitation,Protein purification Cell Tissue Type: Plant extracts |
| FH YACHI (Verified Customer) (02-21-2025) | Highly specific! I obtained a clean Western blot using it. The magnetic properties make it easy to use and fast. Excellent technical support—they answered all my questions. Applications: Western Blot,Immunoprecipitation Cell Tissue Type: Arabidopsis inflorescence stem |
| FH Margaux (Verified Customer) (06-07-2024) | Very efficient, it worked very well with only few microliters Applications: Immunoprecipitation |
| FH Sandra (Verified Customer) (03-28-2024) | The HA-magnetic beads enriched my low abundant bait protein in a higher yield compared to other HA-magnetic beads from another company and in addition with lower background impurities. Elution was performed with 3xHA-peptide and Laemmli buffer. Applications: Immunoprecipitation Cell Tissue Type: Saccharomyces cerevisiae |
Description
Immunoprecipitation of HA-fusion proteins with anti-HA Nanobody conjugated to magnetic agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc.
Publications
- The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice
Journal: Mol Plant | Authors: Dan Hu | Species: CoIP | Application: Mol Plant The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice Authors - Dan Hu View Article - Reliable repurposing of the antibody interactome inside the cell.
Journal: Nat Commun | Authors: Caitlin M. O'Shea | Species: IP | Application: Nat Commun Reliable repurposing of the antibody interactome inside the cell. Authors - Caitlin M. O'Shea View Article - Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes.
Journal: Nucleic Acids Res | Authors: Ya-Chi Nien | Application: Nucleic Acids Res Trans-species microRNAs from the parasitic plant Cuscuta campestris specifically avoid loading onto self Argonautes. Authors - Ya-Chi Nien View Article - Coumarins link iron deficiency to TOR inhibition in plants.
Journal: Curr Biol | Authors: Huikyong Cho | Application: Curr Biol Coumarins link iron deficiency to TOR inhibition in plants. Authors - Huikyong Cho View Article - Maize COMPACT PLANT 3 regulates plant architecture and facilitates high-density planting
Journal: Plant Cell | Authors: Huangjun Sheng | Species: CoIP | Application: Plant Cell Maize COMPACT PLANT 3 regulates plant architecture and facilitates high-density planting Authors - Huangjun Sheng View Article - ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis
Journal: Plant Physiol | Authors: Wan-Ni Wang | Species: IP | Application: Plant Physiol ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis Authors - Wan-Ni Wang View Article
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