HDAC4-specific Polyclonal antibody 16165-1-AP proteintech
$149.00
In stock
SKU
16165-1-AP
| Catalog Number | 16165-1-AP |
| Synonyms | HDAC4, EC:3.5.1.98, HD4, KIAA0288 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, RAW 264.7 cells, HepG2 cells, NIH/3T3 cells, Neuro-2a cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse liver tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | HeLa cells, RAW 264.7 cells, HepG2 cells, NIH/3T3 cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone deacetylase 4 |
| Calculated Molecular Weight | 119 kDa |
| Observed Molecular Weight | 119-140 kDa |
| GenBank Accession Number | BC039904 |
| Gene Symbol | HDAC4 |
| Gene ID (NCBI) | 9759 |
| RRID | AB_2279763 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56524 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HDAC4-specific antibody 16165-1-AP | Download protocol |
| IHC protocol for HDAC4-specific antibody 16165-1-AP | Download protocol |
| IP protocol for HDAC4-specific antibody 16165-1-AP | Download protocol |
| WB protocol for HDAC4-specific antibody 16165-1-AP | Download protocol |
| mouse,human | WB,IP,IF Biochem Pharmacol Mechanistic insights into acacetin-mediated atherosclerosis suppression: targeting HDAC4/p53-regulated ferroptosis signaling pathway. Authors - Ruici Zhao View Article |
| human | WB Hepatology The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma. Authors - Yuan Ji-hang JH KD Validated View Article |
| mouse | WB Biol Trace Elem Res Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG Authors - Lei Chai View Article |
| Citations | 6 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4. Protocols Product Specific Protocols IF protocol for HDAC4-specific antibody 16165-1-AP Download protocol IHC protocol for HDAC4-specific antibody 16165-1-AP Download protocol IP protocol for HDAC4-specific antibody 16165-1-AP Download protocol WB protocol for HDAC4-specific antibody 16165-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.
Journal: Nat Cell Biol | Authors: Yoshihiko Kobayashi | Species: mouse,human | Application: IF - The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma.
Journal: Hepatology | Authors: Yuan Ji-hang JH KD Validated | Species: human | Application: WB - Complex urban atmosphere alters alveolar stem cells niche properties and drives lung fibrosis
Journal: Am J Physiol Lung Cell Mol Physiol | Authors: Randa Belgacemi - Mechanistic insights into acacetin-mediated atherosclerosis suppression: targeting HDAC4/p53-regulated ferroptosis signaling pathway.
Journal: Biochem Pharmacol | Authors: Ruici Zhao | Species: mouse,human | Application: WB,IP,IF - Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta
Journal: J Orthop Translat | Authors: Bin Sun | Species: mouse | Application: WB - Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG
Journal: Biol Trace Elem Res | Authors: Lei Chai | Species: mouse | Application: WB
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