HIP1R Polyclonal antibody 16814-1-AP proteintech
$149.00
In stock
SKU
16814-1-AP
| Catalog Number | 16814-1-AP |
| Synonyms | HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IP, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10353 Product name: Recombinant human HIP1R protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 266-615 aa of BC067085 Sequence: RASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIKPVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPSGSVKDDRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRKQKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKLKEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVKRESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLDTLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQGRLAERVWPPQMQQHH Predict reactive species |
| Full Name | huntingtin interacting protein 1 related |
| Calculated Molecular Weight | 615 aa, 70 kDa, 120 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC067085 |
| Gene Symbol | HIP1R |
| Gene ID (NCBI) | 9026 |
| RRID | AB_2117572 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for HIP1R antibody 16814-1-AP | Download protocol |
| IP protocol for HIP1R antibody 16814-1-AP | Download protocol |
| WB protocol for HIP1R antibody 16814-1-AP | Download protocol |
| human | IHC J Clin Med HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis. Authors - Young Wha Koh View Article |
| Citations | 11 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.
Journal: Cell | Authors: Kazuhiro Koikawa KD Validated | Species: human | Application: WB - Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.
Journal: Nat Cell Biol | Authors: Yang Gao KO Validated | Species: human | Application: WB - Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.
Journal: Nat Biomed Eng | Authors: Han Yao | Species: human | Application: WB - HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.
Journal: Nat Chem Biol | Authors: Huanbin Wang KD Validated | Species: human | Application: WB,IP,IF - The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.
Journal: Sci Adv | Authors: Riyad N H Seervai KD Validated | Species: human | Application: WB - HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.
Journal: J Clin Med | Authors: Young Wha Koh | Species: human | Application: IHC
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