PPIP5K1 Polyclonal antibody 14331-1-AP proteintech
$149.00
In stock
SKU
14331-1-AP
| Catalog Number | 14331-1-AP |
| Synonyms | HISPPD2A, EC:2.7.4.24, InsP6 and PP-IP5 kinase 1, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1, hsVIP1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U-251 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5614 Product name: Recombinant human HISPPD2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC050263 Sequence: MWSLTASEGESTTAHFFLGAGDEGLGTRGIGMRPEESDSELLEDEEDEVPPEPQIIVGICAMTKKSKSKPMTQILERLCRFDYLTVVILGEDVILNEPVENWPSCHCLISFHSKGFPLDKAVAYSKLRNPFLINDLAMQYYIQDRREVYRILQEEGIDLPRYAVLNRDPARPEECNLIEGEDQVEVNGAVFPKPFVEKPVSAEDHNVYIYYPSSAGGGSQRLFRKIGSRSSVYSPESSVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEIRYPVMLTAME Predict reactive species |
| Full Name | histidine acid phosphatase domain containing 2A |
| Calculated Molecular Weight | 160 kDa |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC050263 |
| Gene Symbol | HISPPD2A |
| Gene ID (NCBI) | 9677 |
| RRID | AB_3669185 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PFW1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for PPIP5K1 antibody 14331-1-AP | Download protocol |
| IHC protocol for PPIP5K1 antibody 14331-1-AP | Download protocol |
| WB protocol for PPIP5K1 antibody 14331-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653). Protocols Product Specific Protocols IF protocol for PPIP5K1 antibody 14331-1-AP Download protocol IHC protocol for PPIP5K1 antibody 14331-1-AP Download protocol WB protocol for PPIP5K1 antibody 14331-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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