Di-Methyl-Histone H3 (Lys36) Polyclonal antibody 29202-1-AP proteintech
$149.00
In stock
SKU
29202-1-AP
| Catalog Number | 29202-1-AP |
| Synonyms | Di-Methyl-Histone-H3, Di-Methyl-Histone H3, Di-Methyl Histone-H3 Lys36, Di-Methyl Histone H3 Lys36, Di-Methyl Histone H3 (Lys36) |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, goat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | C2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells |
| Tested Applications — Positive ELISA detected in | peptide |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Positive WB detected in | C2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells |
| Positive ELISA detected in | peptide |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:32000-1:128000 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15-18 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_2918247 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-AP | Download protocol |
| human | WB Cell Signal NSD2-mediated H3K36me2 exacerbates osteoporosis via activation of hoxa2 in bone marrow mesenchymal stem cells Authors - Guanghui He View Article |
| goat, mouse | WB Int J Biol Macromol Disruption of lysine demethylase 2A promotes intramuscular fat deposition and unsaturated fatty acids synthesis via the histone H3 lysine 36 dimethylation-peroxisome proliferator-activated receptor gamma axis. Authors - Binglin Yue View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:8000 ELISA : 1:32000-1:128000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308). Protocols Product Specific Protocols WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- NSD2-mediated H3K36me2 exacerbates osteoporosis via activation of hoxa2 in bone marrow mesenchymal stem cells
Journal: Cell Signal | Authors: Guanghui He | Species: human | Application: WB - Disruption of lysine demethylase 2A promotes intramuscular fat deposition and unsaturated fatty acids synthesis via the histone H3 lysine 36 dimethylation-peroxisome proliferator-activated receptor gamma axis.
Journal: Int J Biol Macromol | Authors: Binglin Yue | Species: goat, mouse | Application: WB
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