HLA class I (HLA-C) Polyclonal antibody 15777-1-AP proteintech
$149.00
In stock
SKU
15777-1-AP
| Catalog Number | 15777-1-AP |
| Synonyms | HLA-C, HLA C, HLA class I histocompatibility antigen, C alpha chain, HLA Cw, HLA Cw12 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA, Cell treatment |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Tested Applications — Positive IP detected in | HepG2 cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8461 Product name: Recombinant human HLA class I (HLA-C) protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 27-305 aa of BC007814 Sequence: SMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTI Predict reactive species |
| Full Name | major histocompatibility complex, class I, C |
| Calculated Molecular Weight | 366 aa, 41 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC007814 |
| Gene Symbol | HLA class I (HLA-C) |
| Gene ID (NCBI) | 3107 |
| RRID | AB_1639892 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10321 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
| IHC protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
| IP protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
| WB protocol for HLA class I (HLA-C) antibody 15777-1-AP | Download protocol |
| human | WB J Reprod Immunol Endometrial microbiota in maternal-fetal tolerance: An in vitro study of extravillous trophoblasts and uNK cells. Authors - Kazuhide Takada View Article |
| mouse | WB Nat Commun Neoantigen enriched biomimetic nanovaccine for personalized cancer immunotherapy Authors - Yuwei Li View Article |
| Citations | 7 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 IF/ICC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250) Protocols Product Specific Protocols IF protocol for HLA class I (HLA-C) antibody 15777-1-AP Download protocol IHC protocol for HLA class I (HLA-C) antibody 15777-1-AP Download protocol IP protocol for HLA class I (HLA-C) antibody 15777-1-AP Download protocol WB protocol for HLA class I (HLA-C) antibody 15777-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis
Journal: Front Pharmacol | Authors: Yanling Wang | Species: human | Application: IHC - Shifts in receptors during submergence of an encephalitic arbovirus
Journal: Nature | Authors: Wanyu Li | Species: human | Application: Cell treatment - HLA is a potent immunoinflammatory target in asymptomatic Alzheimer's disease
Journal: Neuroscience | Authors: Yingwei Zheng | Species: mouse | Application: IF - HLA-C promotes proliferation and cell cycle progression in trophoblast cells
Journal: J Matern Fetal Neonatal Med | Authors: Hong Lv | Species: human | Application: WB - Neoantigen enriched biomimetic nanovaccine for personalized cancer immunotherapy
Journal: Nat Commun | Authors: Yuwei Li | Species: mouse | Application: WB - Endometrial microbiota in maternal-fetal tolerance: An in vitro study of extravillous trophoblasts and uNK cells.
Journal: J Reprod Immunol | Authors: Kazuhide Takada | Species: human | Application: WB
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