HLA-F Monoclonal antibody 66819-1-Ig proteintech
$149.00
In stock
SKU
66819-1-Ig
| Catalog Number | 66819-1-Ig |
| Synonyms | 1B12C2, CDA12, HLA 5.4, HLA CDA12, HLA F |
| Host | Mouse |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | A431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes |
| Tested Applications — Positive IP detected in | Raji cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | Raji cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | A431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6475 Product name: Recombinant human HLA-F protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 21-298 aa of BC062991 Sequence: AGSHSLRYFSTAVSRPGRGEPRYIAVEYVDDTQFLRFDSDAAIPRMEPREPWVEQEGPQYWEWTTGYAKANAQTDRVALRNLLRRYNQSEAGSHTLQGMNGCDMGPDGRLLRGYHQHAYDGKDYISLNEDLRSWTAADTVAQITQRFYEAEEYAEEFRTYLEGECLELLRRYLENGKETLQRADPPKAHVAHHPISDHEATLRCWALGFYPAEITLTWQRDGEEQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPQPLILRWEQS Predict reactive species |
| Full Name | major histocompatibility complex, class I, F |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC062991 |
| Gene Symbol | HLA-F |
| Gene ID (NCBI) | 3134 |
| RRID | AB_2882162 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P30511 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HLA-F antibody 66819-1-Ig | Download protocol |
| IHC protocol for HLA-F antibody 66819-1-Ig | Download protocol |
| WB protocol for HLA-F antibody 66819-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:8000-1:32000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1B12C2 |
| Conjugation | Unconjugated |
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951) Protocols Product Specific Protocols IF protocol for HLA-F antibody 66819-1-Ig Download protocol IHC protocol for HLA-F antibody 66819-1-Ig Download protocol WB protocol for HLA-F antibody 66819-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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