CD45 Polyclonal antibody 20103-1-AP proteintech
$149.00
In stock
SKU
20103-1-AP
| Catalog Number | 20103-1-AP |
| Synonyms | EC:3.1.3.48, GP180, L CA, LCA, L-CA |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (4) |
| Reactivity | human, mouse, rat, pig, canine, bovine, sheep |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, mouse spleen tissue, J774A.1 cells, mouse thymus tissue, rat thymus tissue |
| Tested Applications — Positive IP detected in | mouse spleen tissue |
| Tested Applications — Positive IHC detected in | human colon tissue, human liver cancer tissue, mouse lung tissue, human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human tonsillitis tissue |
| Tested Applications — Positive IF-Fro detected in | mouse spleen tissue |
| Tested Applications — Positive IF/ICC detected in | Jurkat cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:6000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | Jurkat cells, mouse spleen tissue, J774A.1 cells, mouse thymus tissue, rat thymus tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human colon tissue, human liver cancer tissue, mouse lung tissue, human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF-Fro detected in | mouse spleen tissue |
| Positive IF/ICC detected in | Jurkat cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, canine, bovine, sheep |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag13712 Product name: Recombinant human CD45 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 550-750 aa of BC014239 Sequence: KIYDLHKKRSCNLDEQQELVERDDEKQLMNVEPIHADILLETYKRKIADEGRLFLAEFQSIPRVFSKFPIKEARKPFNQNKNRYVDILPYDYNRVELSEINGDAGSNYINASYIDGFKEPRKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEEGTRAFGDVVVKINQHKRCPDYIIQKLNIV Predict reactive species |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Calculated Molecular Weight | 1304 aa, 147 kDa |
| Observed Molecular Weight | 180-220 kDa |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2716813 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD45 antibody 20103-1-AP | Download protocol |
| IHC protocol for CD45 antibody 20103-1-AP | Download protocol |
| IP protocol for CD45 antibody 20103-1-AP | Download protocol |
| WB protocol for CD45 antibody 20103-1-AP | Download protocol |
| mouse | IF Cancer Commun (Lond) Pivotal roles of tumor-draining lymph nodes in the abscopal effects from combined immunotherapy and radiotherapy Authors - Zhaoyun Liu View Article |
| human | IF Drug Resist Updat MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance Authors - GuoSheng Wang View Article |
| Alice (Verified Customer) (09-11-2026) | Used on iPSC-derived immune cells. The antibody showed good specificity and staining quality. Applications: Immunofluorescence Primary Antibody Dilution: 1:250 Cell Tissue Type: iPSC-derived immune cells |
| Christin (Verified Customer) (06-14-2025) | Worked very well in ICC for iPSC-derived macrophages Applications: Immunofluorescence Primary Antibody Dilution: 1:250 Cell Tissue Type: iPSC-derived macrophages |
| Alexandru (Verified Customer) (11-13-2023) | Works great on paraffin embedded tissue section (after antigen retrival!) Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: Mouse ventricle |
| Kenzo (Verified Customer) (01-09-2023) | This antibody worked well as a hematopoietic cell marker for IF on tissue sections. Applications: Immunofluorescence Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse tonsil |
| Emma (Verified Customer) (02-22-2022) | Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval) Applications: Immunofluorescence Primary Antibody Dilution: 1:25 Cell Tissue Type: Prostate |
| Citations | 168 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:1000-1:6000 IF-P : 1:50-1:500 IF-FRO : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Protein tyrosine phosphatase receptor type C (PTPRC) is also known as CD45 antigen, which was originally called leukocyte common antigen (LCA). CD45 is an enzyme required for T-cell activation through the antigen receptor consisting of two intracellular phosphatase domains, a transmembrane domain and an extracellular domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 via an interaction through the first PTPase domain, which is the only domain to possess intrinsic kinase activity. However, both domains are required for appropriate phosphatase and T-cell antigen receptor activity (PMID: 8076596). What is the molecular weight of CD45? The molecular weight of CD45 is a 180-240 kDa. What are the isoforms of CD45? Six different human isoforms of CD45 have been isolated, containing all three exons (ABC isoform), two of the three exons (AB and BC isoforms), only one exon (A isoform or B isoform), or no exons (O isoform). All of the isoforms have the same eight amino acids at their amino-terminus (PMID: 12412720). What is the tissue specificity of CD45? CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets (PMID: 7803253). What are the post-translational modifications of CD45? CD45 can be heavily N- and O-glycosylated (PMID: 1702721). What is CD45's involvement in disease? Defects in CD45 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID); a severe form of SCID. It is classed by the impairment of both humoral and cell-mediated immunity, leukopenia, and the low or absent levels of antibodies (PMID: 10700239). Also, genetic variants of CD45 have a role in susceptibility to multiple sclerosis (PMID: 12864992). Protocols Product Specific Protocols IF protocol for CD45 antibody 20103-1-AP Download protocol IHC protocol for CD45 antibody 20103-1-AP Download protocol IP protocol for CD45 antibody 20103-1-AP Download protocol WB protocol for CD45 antibody 20103-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses
Journal: Cell Res | Authors: Shufen Li | Species: mouse | Application: IHC - Pivotal roles of tumor-draining lymph nodes in the abscopal effects from combined immunotherapy and radiotherapy
Journal: Cancer Commun (Lond) | Authors: Zhaoyun Liu | Species: mouse | Application: IF - Niche inflammatory signals control oscillating mammary regeneration and protect stem cells from cytotoxic stress
Journal: Cell Stem Cell | Authors: Chunye Liu - SIRT1 Inhibits T-cell Infiltration and Tertiary Lymphoid Structure Formation to Promote Radioimmunotherapy Resistance.
Journal: Cancer Res | Authors: Yunxing Shi - MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance
Journal: Drug Resist Updat | Authors: GuoSheng Wang | Species: human | Application: IF - Engineering high-affinity dual targeting cellular nanovesicles for optimised cancer immunotherapy
Journal: J Extracell Vesicles | Authors: Luyao Zhang | Application: IF
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