HMG20A Polyclonal antibody 12085-2-AP proteintech
$149.00
In stock
SKU
12085-2-AP
| Catalog Number | 12085-2-AP |
| Synonyms | HMGX1, HMG domain-containing protein HMGX1, HMG domain-containing protein 1, HMG box-containing protein 20A, HMG box containing protein 20A |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, HEK-293 cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Tested Applications — Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | Jurkat cells, HEK-293 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2718 Product name: Recombinant human HMG20A protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-347 aa of BC021959 Sequence: MENLMTSSTLPPLFADEDGSKESNDLATTGLNHPEVPYSSGATSSTNNPEFVEDLSQGQLLQSESSNAAEGNEQRHEDEQRSKRGGWSKGRKRKKPLRDSNAPKSPLTGYVRFMNERREQLRAKRPEVPFPEITRMLGNEWSKLPPEEKQRYLDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRTAVEKLEVDVIQERSRNTVLQQHLETLRQVLTSSFASMPLPGSGETPTVDTIDSYMNRLHSIILANPQDNENFIATVREVVNRLDR Predict reactive species |
| Full Name | high-mobility group 20A |
| Calculated Molecular Weight | 347 aa, 40 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC021959 |
| Gene Symbol | HMG20A |
| Gene ID (NCBI) | 10363 |
| RRID | AB_2117587 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NP66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HMG20A antibody 12085-2-AP | Download protocol |
| IHC protocol for HMG20A antibody 12085-2-AP | Download protocol |
| IP protocol for HMG20A antibody 12085-2-AP | Download protocol |
| WB protocol for HMG20A antibody 12085-2-AP | Download protocol |
| human | IHC Biochim Biophys Acta Interactome of the inhibitory isoform of the nuclear transporter Importin 13. Authors - Shadma Fatima View Article |
| mouse | WB,IF bioRxiv Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7 Authors - Chuang Li View Article |
| Citations | 11 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation Protocols Product Specific Protocols IF protocol for HMG20A antibody 12085-2-AP Download protocol IHC protocol for HMG20A antibody 12085-2-AP Download protocol IP protocol for HMG20A antibody 12085-2-AP Download protocol WB protocol for HMG20A antibody 12085-2-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics
Journal: Nat Commun | Authors: Mai Abdel Mouti | Species: human | Application: WB - The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs
Journal: Nat Commun | Authors: Andreas Herchenröther KD Validated | Species: human | Application: WB,IP - LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.
Journal: EMBO Mol Med | Authors: Lukas Leiendecker | Species: human | Application: WB - Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.
Journal: Cancer Cell Int | Authors: Yu Fu | Species: mouse | Application: WB - Interactome of the inhibitory isoform of the nuclear transporter Importin 13.
Journal: Biochim Biophys Acta | Authors: Shadma Fatima | Species: human | Application: IHC - Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7
Journal: bioRxiv | Authors: Chuang Li | Species: mouse | Application: WB,IF - Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7
Journal: PLoS Pathog | Authors: Chuang Li | Species: mouse | Application: WB,IF - Interactome of the inhibitory isoform of the nuclear transporter Importin 13.
Journal: Biochim Biophys Acta | Authors: Shadma Fatima | Species: human | Application: IHC - HMG20A was identified as a key enhancer driver associated with DNA damage repair in oral squamous cell carcinomas
Journal: BMC Oral Health | Authors: Li Na | Species: human | Application: WB - The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.
Journal: Biochim Biophys Acta | Authors: Davies Rebecca G RG | Species: mouse | Application: WB,IHC,IF,IP - Legionella pneumophila exploits the endo-lysosomal network for phagosome biogenesis by co-opting SUMOylated Rab7
Journal: bioRxiv | Authors: Chuang Li | Species: mouse | Application: WB,IF
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
