HP1BP3 Recombinant monoclonal antibody 86974-2-RR proteintech
$149.00
In stock
SKU
86974-2-RR
| Catalog Number | 86974-2-RR |
| Synonyms | Heterochromatin protein 1-binding protein 3, HP1 BP74, Protein HP1 BP74, Protein HP1-BP74 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, EC109 cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, EC109 cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag18709 Product name: Recombinant human HP1BP3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-302 aa of BC045660 Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK Predict reactive species |
| Full Name | heterochromatin protein 1, binding protein 3 |
| Calculated Molecular Weight | 553 aa, 61 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC045660 |
| Gene Symbol | HP1BP3 |
| Gene ID (NCBI) | 50809 |
| RRID | AB_3745247 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5SSJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HP1BP3 antibody 86974-2-RR | Download protocol |
| WB protocol for HP1BP3 antibody 86974-2-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:500-1:2000 IF/ICC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 252095F2 |
| Conjugation | Unconjugated |
HP1BP3 (heterochromatin protein 1-binding protein 3) is an evolutionarily conserved nuclear protein that functions as a non-canonical linker histone. It is broadly expressed in most vertebrate tissues and is structurally related to the histone H1 family, containing three globular domains and a highly basic C-terminal tail reminiscent of H1 proteins . Unique to HP1BP3 are an acidic N-terminal stretch and a consensus HP1-binding motif (PxVxL), through which it associates with heterochromatin protein 1 (HP1α/β) and is enriched at perinucleolar heterochromatin regions marked by H3K9me3. Functionally, HP1BP3 acts as a modulator of gene expression and genome stability. Alternative splicing was reported to produce 4 different isoforms of HP1BP3, the calculated MW corresponding to 61, 57, 44, 15 kDa. Protocols Product Specific Protocols IF protocol for HP1BP3 antibody 86974-2-RR Download protocol WB protocol for HP1BP3 antibody 86974-2-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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