ENO1/2/3 Recombinant monoclonal antibody 81504-1-RR proteintech
$149.00
In stock
SKU
81504-1-RR
| Catalog Number | 81504-1-RR |
| Synonyms | ENO1, 2-phospho-D-glycerate hydro-lyase, Alpha-enolase, C-myc promoter-binding protein, EC:4.2.1.11 |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, FC (Intra), ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, mouse brain tissue, rat brain tissue, pig brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Tested Applications — Positive FC (Intra) detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells, MCF-7 cells, Jurkat cells, mouse brain tissue, rat brain tissue, pig brain tissue |
| Positive IHC detected in | mouse brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag1692 Product name: Recombinant human ENO1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-434 aa of BC015641 Sequence: SILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK Predict reactive species |
| Full Name | enolase 1, (alpha) |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC015641 |
| Gene Symbol | ENO1 |
| Gene ID (NCBI) | 2023 |
| ENSEMBL Gene ID | ENSG00000074800 |
| RRID | AB_2935557 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P06733 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for ENO1/2/3 antibody 81504-1-RR | Download protocol |
| IF protocol for ENO1/2/3 antibody 81504-1-RR | Download protocol |
| IHC protocol for ENO1/2/3 antibody 81504-1-RR | Download protocol |
| WB protocol for ENO1/2/3 antibody 81504-1-RR | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:50-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 6E24 |
| Conjugation | Unconjugated |
Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3. Protocols Product Specific Protocols FC protocol for ENO1/2/3 antibody 81504-1-RR Download protocol IF protocol for ENO1/2/3 antibody 81504-1-RR Download protocol IHC protocol for ENO1/2/3 antibody 81504-1-RR Download protocol WB protocol for ENO1/2/3 antibody 81504-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- HRASLS2 promotes the growth and glycolysis of pancreatic cancer by enhancing the stability of ASPH
Journal: Naunyn Schmiedebergs Arch Pharmacol | Authors: Ying Ling
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