FLI1 Recombinant monoclonal antibody 85931-1-RR proteintech
$149.00
In stock
SKU
85931-1-RR
| Catalog Number | 85931-1-RR |
| Synonyms | FLI 1, FLI-1, Friend leukemia virus integration 1, Proto-oncogene Fli-1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, HL-60 cells, U-937 cells, A20 cells, RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | human tonsil tissue, human breast cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HEL cells, Jurkat cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | Jurkat cells, HL-60 cells, U-937 cells, A20 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsil tissue, human breast cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEL cells, Jurkat cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag36169 Product name: Recombinant human FLI1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 103-452 aa of BC010115 Sequence: NSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY Predict reactive species |
| Full Name | Friend leukemia virus integration 1 |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC010115 |
| Gene Symbol | FLI1 |
| Gene ID (NCBI) | 2313 |
| RRID | AB_3744482 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q01543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for FLI1 antibody 85931-1-RR | Download protocol |
| IHC protocol for FLI1 antibody 85931-1-RR | Download protocol |
| WB protocol for FLI1 antibody 85931-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:500-1:2000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250294F12 |
| Conjugation | Unconjugated |
Friend leukemia virus integration1 (FLI1) that is also termed EWSR2 encodes a sequence-specific transcriptional activator involved in Ewing's sarcoma (EWS), a malignant small, round, blue cell tumour. It is a rare disease in which cancer cells are found in the bone or in soft tissue. The most common areas in which it occurs are the pelvis, the femur, the humerus, the ribs and clavicle. EWS occurs most frequently in teenagers and young adults. FLI1 protein may act as an aberrant transcription factor, but exact mechanisms of oncogenesis remains unknown. Protocols Product Specific Protocols IF protocol for FLI1 antibody 85931-1-RR Download protocol IHC protocol for FLI1 antibody 85931-1-RR Download protocol WB protocol for FLI1 antibody 85931-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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